Evidence map›Paper›PMID 30385787›Full record

ArticleScientific reports2018

Overexpression of CD98 in intestinal epithelium dysregulates miRNAs and their targeted proteins along the ileal villus-crypt axis.

Moon K Han, Mark Baker, Yuchen Zhang, Chunhua Yang, Mingzhen Zhang, Pallavi Garg, Emilie Viennois, Didier Merlin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Moon K HanInstitute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Center for Inflammation, Immunity and Infection, Digestive Disease Research Group, Georgia State University, Atlanta, 30303, USA. mkim15@gsu.edu.
Mark BakerInstitute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Center for Inflammation, Immunity and Infection, Digestive Disease Research Group, Georgia State University, Atlanta, 30303, USA.
Yuchen ZhangInstitute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Center for Inflammation, Immunity and Infection, Digestive Disease Research Group, Georgia State University, Atlanta, 30303, USA.
Chunhua YangInstitute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Center for Inflammation, Immunity and Infection, Digestive Disease Research Group, Georgia State University, Atlanta, 30303, USA.
Mingzhen ZhangInstitute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Center for Inflammation, Immunity and Infection, Digestive Disease Research Group, Georgia State University, Atlanta, 30303, USA.
Pallavi GargInstitute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Center for Inflammation, Immunity and Infection, Digestive Disease Research Group, Georgia State University, Atlanta, 30303, USA.
Emilie ViennoisInstitute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Center for Inflammation, Immunity and Infection, Digestive Disease Research Group, Georgia State University, Atlanta, 30303, USA.
Didier MerlinInstitute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Center for Inflammation, Immunity and Infection, Digestive Disease Research Group, Georgia State University, Atlanta, 30303, USA.
Georgia State University · US

Funding

ROLE OF CD98 IN INTESTINAL PERMEABILITYR01DK071594 · NIDDK · GEORGIA STATE UNIVERSITY · PI MERLIN, DIDIER · 2006 to 2017
$3.3M
Fecal exosomes as a source of miRNA biomarkers for diagnosing the degree of colitis and as a drug delivery system to reduce colitisR01DK116306 · NIDDK · GEORGIA STATE UNIVERSITY · PI DENNING, TIMOTHY L, GEWIRTZ, ANDREW T · 2017 to 2020
$1.8M
Novel Therapeutic Approaches For Treatment of Intestinal InflammationR01DK107739 · NIDDK · GEORGIA STATE UNIVERSITY · PI DENNING, TIMOTHY L, MERLIN, DIDIER · 2017 to 2020
$1.4M
Small intestinal PepT1 expression plays a critical role in maintaining intestinal homeostasis and in shaping the gut microbiotaI01BX002526 · VA · VETERANS HEALTH ADMINISTRATION · PI MERLIN, DIDIER · 2014 to 2024
–
BLRD VA I01 BX002526NIDDK NIH HHS R01 DK071594NIDDK NIH HHS R01 DK107739NIDDK NIH HHS R01 DK116306
6 · The paper itself

Abstract

CD98 has been implicated in the experimental model of inflammatory bowel disease. We have previously shown that IEC-specific overexpression of CD98 mediates intestinal inflammation and intestinal epithelial barrier dysfunction. Mice overexpressing CD98 exhibited severe colitis and a greater susceptibility to CAC. Here we demonstrated CD98 overexpression to dysregulate homeostatic gradient profile of miRNA and protein expression along the ileal villus-crypt axis. Using miRNA-target gene prediction module, we observed differentially expressed miRNAs to target proteins of villus and crypt profoundly affected by CD98 overexpression. We have utilized online bioinformatics as methods to further scrutinize the biological meanings of miRNA-target data. We identified significant interactions among the differentially regulated proteins targeted by altered miRNAs in Tg mice. The biological processes affected by the predicted targets of miRNAs deviate from the homeostatic functions of the miRNA-gene-protein axis of the wildtype mice. Our results emphasize a dynamic perturbation of miRNA and protein expression in villus-crypt axis contributing to potential biological consequences of altering CD98 expression. Our findings also suggest the need for a consideration of arrays of interacting biological entities (i.e. miRNAs-mRNAs, protein-protein interaction) or a combination comparison for a better understanding of the disease pathology which is necessary for an effective therapeutic target development.

Indexed as

Gene ExpressionGene Expression RegulationRNA InterferenceAnimalsComputational BiologyFemaleFusion Regulatory Protein-1Gene Expression ProfilingGene Regulatory NetworksIleumIntestinal MucosaMiceMicroRNAsFusion Regulatory Protein-1MicroRNAs

Identifiers

PMID30385787
PMCPMC6212412
OpenAlexW2898591202

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.