ReviewCold Spring Harbor perspectives in biology2018
Quantifying Single mRNA Translation Kinetics in Living Cells.
Review in Cold Spring Harbor perspectives in biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- Real-time single-molecule imaging in zebrafish embryos uncovers noncanonical translation.Science advances · 2026Article
- Single-mRNA imaging and modeling reveal coupled translation initiation and elongation rates.eLife · 2026Article
- Bridging single-molecule and genome-wide studies of cellular mRNA translation.RNA (New York, N.Y.) · 2026Review
- Combined statistical-biophysical modeling links ion channel genes to physiology of cortical neuron types.Patterns (New York, N.Y.) · 2025Article
- All Probes Plasmids (APPs) for multicolor and long-term tracking of single-mRNA translation dynamics.Molecular biology of the cell · 2025Article
- Imaging Translation in Early Embryo Development.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Imaging and Quantifying Ribosomal Frameshifting Dynamics with Single-RNA Precision in Live Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Direct observation of translational activation by a ribonucleoprotein granule.Nature cell biology · 2024Article
- Translation Dynamics of Single mRNAs in Live Cells.Annual review of biophysics · 2024Review
- Central dogma rates in human mitochondria.Human molecular genetics · 2024Review
- A kinetic dichotomy between mitochondrial and nuclear gene expression processes.Molecular cell · 2024Article
- Article
- Decomposing bulk signals to reveal hidden information in processive enzyme reactions: A case study in mRNA translation.PLoS computational biology · 2024Article
- A kinetic dichotomy between mitochondrial and nuclear gene expression drives OXPHOS biogenesis.bioRxiv : the preprint server for biology · 2023Article
- Deciphering molecular mechanisms of phase separation in RNA biology by single-molecule biophysical technologies.Acta biochimica et biophysica Sinica · 2023Article
- Cap-dependent translation initiation monitored in living cells.Nature communications · 2022Article
- Regulation and outcomes of localized RNA translation.Wiley interdisciplinary reviews. RNA · 2022Review
- Formulation of Lipid-Free Polymeric Mesoscale Nanoparticles Encapsulating mRNA.Pharmaceutical research · 2022Article
- Imaging translational control by Argonaute with single-molecule resolution in live cells.Nature communications · 2022Article
- Single-Molecule Imaging of mRNA Interactions with Stress Granules.Methods in molecular biology (Clifton, N.J.) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
One of the last hurdles to quantifying the full central dogma of molecular biology in living cells with single-molecule resolution has been the imaging of single messenger RNA (mRNA) translation. Here we describe how recent advances in protein tagging and imaging technologies are being used to precisely visualize and quantify the synthesis of nascent polypeptide chains from single mRNA in living cells. We focus on recent applications of repeat-epitope tags and describe how they enable quantification of single mRNA ribosomal densities, translation initiation and elongation rates, and translation site mobility and higher-order structure. Together with complementary live-cell assays to monitor translation using fast-maturing fluorophores and mRNA-binding protein knockoff, single-molecule studies are beginning to uncover striking and unexpected heterogeneity in gene expression at the level of translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.