Evidence map›Paper›PMID 30385505›Full record

ArticleThe Journal of biological chemistry2018

Galectin binding to cells and glycoproteins with genetically modified glycosylation reveals galectin-glycan specificities in a natural context.

Mathias Ingemann Nielsen, John Stegmayr, Oliver C Grant, Zhang Yang, Ulf J Nilsson, Irene Boos, Michael C Carlsson, Robert J Woods, Carlo Unverzagt, Hakon Leffler and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 87 citations in OpenAlex.

  1. Review
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  6. Galectin-9-An Emerging Glyco-Immune Checkpoint Target for Cancer Therapy.International journal of molecular sciences · 2025
    Review
  7. Review
  8. Article
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  10. Implications of Mucin-TypeMolecules (Basel, Switzerland) · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 4 countries.

Mathias Ingemann NielsenFrom the Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark.
John Stegmayrthe Division for Microbiology, Immunology and Glycobiology (MIG), Department of Laboratory Medicine, Faculty of Medicine, Lund University, 22100 Lund, Sweden.
Oliver C Grantthe Complex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602.
Zhang YangFrom the Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark.
Ulf J Nilssonthe Centre for Analysis and Synthesis, Department of Chemistry, Lund University, 22100 Lund, Sweden, and.
Irene Boosthe Bioorganische Chemie, Universität Bayreuth, Gebäude NW I, 95440 Bayreuth, Germany.
Michael C CarlssonFrom the Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark,; the Division for Microbiology, Immunology and Glycobiology (MIG), Department of Laboratory Medicine, Faculty of Medicine, Lund University, 22100 Lund, Sweden.
Robert J Woodsthe Complex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602.
Carlo Unverzagtthe Bioorganische Chemie, Universität Bayreuth, Gebäude NW I, 95440 Bayreuth, Germany.
Hakon Lefflerthe Division for Microbiology, Immunology and Glycobiology (MIG), Department of Laboratory Medicine, Faculty of Medicine, Lund University, 22100 Lund, Sweden.
Hans H WandallFrom the Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark,. Electronic address: hhw@sund.ku.dk.
University of Copenhagen · DKLund University · SEUniversity of Bayreuth · DEUniversity of Georgia · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Galectins compose a protein family defined by a conserved sequence motif conferring affinity for β-galactose-containing glycans. Moreover, galectins gain higher affinity and fine-tune specificity by glycan interactions at sites adjacent to their β-galactoside-binding site, as revealed by extensive testing against panels of purified glycans. However, in cells, galectins bind glycans on glycoproteins and glycolipids in the context of other cellular components, such as at the cell surface. Because of difficulties in characterizing natural cellular environments, we currently lack a detailed understanding of galectin-binding specificities in the cellular context. To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to

Indexed as

AnimalsCHO CellsCricetulusGalectinsGlycosylationHumansPolysaccharidesProtein DomainsGalectinsPolysaccharidescarbohydrate-binding proteingalectinGalNAcgenetic engineeringglycanglycationglycobiologyglycolipidglycoproteinLacNAcmolecular geneticssialic acid

Identifiers

PMID30385505
PMCPMC6311502
OpenAlexW2899401674

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.