Evidence map›Paper›PMID 30384153›Full record

ArticleArchives of oral biology2019

Salivary gland cell aggregates are derived from self-organization of acinar lineage cells.

Jomy J Varghese, M Eva Hansen, Azmeer Sharipol, Matthew H Ingalls, Martha A Ormanoski, Shawn D Newlands, Catherine E Ovitt, Danielle S W Benoit

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of oral biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Jomy J VargheseDepartment of Biomedical Engineering, University of Rochester, United States.
M Eva HansenDepartment of Biomedical Engineering, University of Rochester, United States.
Azmeer SharipolDepartment of Biomedical Engineering, University of Rochester, United States.
Matthew H IngallsDepartment of Biomedical Genetics, University of Rochester, United States.
Martha A OrmanoskiDepartment of Biomedical Engineering, Cornell University, United States.
Shawn D NewlandsDepartment of Otolaryngology, University of Rochester, United States; Wilmot Cancer Institute, University of Rochester, United States; Department of Neuroscience, University of Rochester, United States.
Catherine E OvittDepartment of Biomedical Genetics, University of Rochester, United States; Center for Oral Biology, University of Rochester, United States. Electronic address: catherine_ovitt@urmc.rochester.edu.
Danielle S W BenoitDepartment of Biomedical Engineering, University of Rochester, United States; Department of Biomedical Genetics, University of Rochester, United States; Center for Oral Biology, University of Rochester, United States; Center for Musculoskeletal Research, University of Rochester, United States; Department of Orthopaedics, University of Rochester, United States; Department of Chemical Engineering, University of Rochester, United States. Electronic address: benoit@bme.rochester.edu.
University of Rochester · USCornell University · USUniversity of Rochester Medical Center · US

Funding

Training Program in Oral ScienceT90DE021985 · NIDCR · UNIVERSITY OF ROCHESTER · PI OVITT, CATHERINE, YULE, DAVID I · 2011 to 2021
$5.6M
Engineered salivary gland tissue chips (Administrative Supplement)UH3DE027695 · NIDCR · UNIVERSITY OF ROCHESTER · PI BENOIT, DANIELLE S., DELOUISE, LISA A · 2019 to 2021
$3.0M
Engineered salivary gland tissue chipsUG3DE027695 · NIDCR · UNIVERSITY OF ROCHESTER · PI BENOIT, DANIELLE S., DELOUISE, LISA A · 2017 to 2018
$1.5M
Salivary gland-specific radioprotectionR56DE025098 · NIDCR · UNIVERSITY OF ROCHESTER · PI BENOIT, DANIELLE S., OVITT, CATHERINE · 2016 to 2016
$531k
Engineered Nanoparticles to Radioprotect Salivary TissueF30CA206296 · NCI · UNIVERSITY OF ROCHESTER · PI VARGHESE, JOMY JO · 2016 to 2019
$192k
NCI NIH HHS F30 CA206296NIDCR NIH HHS R56 DE025098NIDCR NIH HHS T90 DE021985NIDCR NIH HHS UG3 DE027695NIDCR NIH HHS UH3 DE027695
6 · The paper itself

Abstract

objectiveThe objective of this study was to characterize the mechanism by which salivary gland cells (SGC) aggregate in vitro.

designTimelapse microscopy was utilized to analyze the process of salivary gland aggregate formation using both primary murine and human salivary gland cells. The role of cell density, proliferation, extracellular calcium, and secretory acinar cells in aggregate formation was investigated. Finally, the ability of cells isolated from irradiated glands to form aggregates was also evaluated.

resultsSalivary gland cell self-organization rather than proliferation was the predominant mechanism of aggregate formation in both primary mouse and human salivary gland cultures. Aggregation was found to require extracellular calcium while acinar lineage cells account for ∼80% of the total aggregate cell population. Finally, aggregation was not impaired by irradiation.

conclusionsThe data reveal that aggregation occurs as a result of heterogeneous salivary gland cell self-organization rather than from stem cell proliferation and differentiation, contradicting previous dogma. These results suggest a re-evaluation of aggregate formation as a criterion defining salivary gland stem cells.

Indexed as

Acinar CellsAnimalsCalciumCell CountCell DifferentiationCell ProliferationCells, CulturedHumansMiceSalivary GlandsCalciumAcinar cellsAggregatesRadiation damageSalivary gland

Identifiers

PMID30384153
PMCPMC6323641
OpenAlexW2896566779

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.