ArticleScientific reports2018
Murine Oviductosomes (OVS) microRNA profiling during the estrous cycle: Delivery of OVS-borne microRNAs to sperm where miR-34c-5p localizes at the centrosome.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Extracellular vesicles in reproductive biology and disorders: a comprehensive review.Frontiers in endocrinology · 2025Pooled it
- [The role of extracellular vesicles in early embryo development and their application in assisted reproductive technologies].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2025Review
- Parental noncoding RNA expression dynamics across sperm, oocyte, and zygote.NAR genomics and bioinformatics · 2025Article
- Review
- Engineered extracellular vesicles: a breakthrough approach to overcoming sperm cryopreservation challenges.Reproductive biology and endocrinology : RB&E · 2025Review
- Review
- Extracellular vesicles in female reproduction: from basic research to application.Animal reproduction · 2025Review
- Proteomic analysis and in vivo visualization of extracellular vesicles from mouse oviducts during pre-implantation embryo development.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Exploring the potential of in vitro extracellular vesicle generation in reproductive biology.Journal of extracellular biology · 2024Review
- Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality.BMC genomics · 2024Article
- Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare.Scientific reports · 2022Article
- Prenatal Exposure to Delta-9-tetrahydrocannabinol (THC) Alters the Expression of miR-122-5p and Its TargetInternational journal of molecular sciences · 2022Article
- The role of extracellular vesicles in animal reproduction and diseases.Journal of animal science and biotechnology · 2022Review
- Mysteries and unsolved problems of mammalian fertilization and related topics.Biology of reproduction · 2022Review
- An interactive analysis of the mouse oviductal miRNA profiles.Frontiers in cell and developmental biology · 2022Article
- Messenger roles of extracellular vesicles during fertilization of gametes, development and implantation: Recent advances.Frontiers in cell and developmental biology · 2022Review
- Extracellular vesicles in reproduction and pregnancy.Extracellular vesicles and circulating nucleic acids · 2022Review
- Engineered EV-Mimetic Nanoparticles as Therapeutic Delivery Vehicles for High-Grade Serous Ovarian Cancer.Cancers · 2021Review
- Role and Modulation of TRPV1 in Mammalian Spermatozoa: An Updated Review.International journal of molecular sciences · 2021Review
- Steroidal Regulation of Oviductal microRNAs Is Associated with microRNA-Processing in Beef Cows.International journal of molecular sciences · 2021Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Oviductosomes (OVS) are nano-sized extracellular vesicles secreted in the oviductal luminal fluid by oviductal epithelial cells and known to be involved in sperm capacitation and fertility. Although they have been shown to transfer encapsulated proteins to sperm, cargo constituents other than proteins have not been identified. Using next-generation sequencing, we demonstrate that OVS are carriers of microRNAs (miRNAs), with 272 detected throughout the estrous cycle. Of the 50 most abundant, 6 (12%) and 2 (4%) were expressed at significantly higher levels (P < 0.05) at metestrus/diestrus and proestrus/estrus. RT-qPCR showed that selected miRNAs are present in oviductal epithelial cells in significantly (P < 0.05) lower abundance than in OVS, indicating selective miRNA packaging. The majority (64%) of the top 25 OVS miRNAs are present in sperm. These miRNAs' potential target list is enriched with transcription factors, transcription regulators, and protein kinases and there are several embryonic developmentally-related genes. Importantly, OVS can deliver to sperm miRNAs, including miR-34c-5p which is essential for the first cleavage and is solely sperm-derived in the zygote. Z-stack of confocal images of sperm co-incubated with OVS loaded with labeled miRNAs showed the intracellular location of the delivered miRNAs. Interestingly, individual miRNAs were predominantly localized in specific head compartments, with miR-34c-5p being highly concentrated at the centrosome where it is known to function. These results, for the first time, demonstrate OVS' ability to contribute to the sperm's miRNA repertoire (an important role for solely sperm-derived zygotic miRNAs) and the physiological relevance of an OVS-borne miRNA that is delivered to sperm.
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