Evidence map›Paper›PMID 30382099›Full record

ArticleNature communications2018

Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif.

Sandip A Shelke, Yaming Shao, Artur Laski, Deepak Koirala, Benjamin P Weissman, James R Fuller, Xiaohong Tan, Tudor P Constantin, Alan S Waggoner, Marcel P Bruchez and 2 more

Abstract read
In one paragraph

Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

  1. Article
  2. Article
  3. Structure-based insights into fluorogenic RNA aptamers.Acta biochimica et biophysica Sinica · 2024
    Review
  4. Review
  5. Article
  6. Structure of saguaro cactus virus 3' translational enhancer mimics 5' cap for eIF4E binding.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Structural analyses of an RNA stability element interacting with poly(A).Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Sandip A ShelkeDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, 60637, USA.
Yaming ShaoDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, 60637, USA.
Artur LaskiDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, 60637, USA.
Deepak KoiralaDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, 60637, USA.
Benjamin P WeissmanDepartment of Chemistry, The University of Chicago, Chicago, IL, 60637, USA.
James R FullerDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, 60637, USA.ORCID http://orcid.org/0000-0002-9029-0923
Xiaohong TanCenter for Nucleic Acids Science and Technology, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.
Tudor P ConstantinDepartment of Chemistry, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.
Alan S WaggonerDepartment of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.ORCID http://orcid.org/0000-0002-5188-4075
Marcel P BruchezDepartment of Chemistry, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.ORCID http://orcid.org/0000-0002-7370-4848
Bruce A ArmitageCenter for Nucleic Acids Science and Technology, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.
Joseph A PiccirilliDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, 60637, USA. jpicciri@uchicago.edu.ORCID http://orcid.org/0000-0002-0541-6270

Funding

Overall EvaluationP41GM103403 · NIGMS · CORNELL UNIVERSITY · PI EALICK, STEVEN E · 2012 to 2017
$14.3M
Predoctoral Training Program in Chemistry & BiologyT32GM008720 · NIGMS · UNIVERSITY OF CHICAGO · PI PICCIRILLI, JOSEPH ANTHONY · 1999 to 2020
$4.7M
CHAPERONE-ASSISTED RNA CRYSTALLOGRAPHY - Resubmission 01R01GM102489 · NIGMS · UNIVERSITY OF CHICAGO · PI PICCIRILLI, JOSEPH ANTHONY · 2013 to 2021
$2.9M
Investigating the Catalytic Mechanism of the HDV RibozymeR01AI081987 · NIAID · UNIVERSITY OF CHICAGO · PI PICCIRILLI, JOSEPH ANTHONY · 2010 to 2014
$1.8M
NIAID NIH HHS R01 AI081987NIGMS NIH HHS P41 GM103403NIGMS NIH HHS R01 GM102489NIGMS NIH HHS T32 GM008720
6 · The paper itself

Abstract

The DIR2s RNA aptamer, a second-generation, in-vitro selected binder to dimethylindole red (DIR), activates the fluorescence of cyanine dyes, DIR and oxazole thiazole blue (OTB), allowing detection of two well-resolved emission colors. Using Fab BL3-6 and its cognate hairpin as a crystallization module, we solved the crystal structures of both the apo and OTB-SO

Indexed as

G-QuadruplexesNucleotide MotifsAptamers, NucleotideBinding SitesCrystallography, X-RayFluorescent DyesImmunoglobulin Fab FragmentsAptamers, NucleotideFluorescent DyesImmunoglobulin Fab Fragments

Identifiers

PMID30382099
PMCPMC6208384

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.