ArticleProceedings of the National Academy of Sciences of the United States of America2018
Silencing of retrotransposon-derived imprinted gene RTL1 is the main cause for postimplantational failures in mammalian cloning.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Epigenetic Drift and the Generational Limit of Serial Somatic Cell Nuclear Transfer in Pigs.Animals : an open access journal from MDPI · 2026Review
- Generating high-quality porcine iPSCs with the new medium cocktail LACID.Stem cell reports · 2026Article
- The progress of induced pluripotent stem cells derived from pigs: a mini review of recent advances.Frontiers in cell and developmental biology · 2024Review
- Expression patterns and DNA methylation profile ofAnimal biotechnology · 2023Article
- Generation of stable integration-free pig induced pluripotent stem cells under chemically defined culture condition.Cell proliferation · 2023Article
- Imprinted Dlk1-Gtl2 cluster miRNAs are potential epigenetic regulators of lamb fur quality.BMC genomics · 2023Article
- Epigenetic mechanism of Gtl2-miRNAs causes the primitive sheep characteristics found in purebred Merino sheep.Cell & bioscience · 2023Article
- - Invited Review - Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm.Animal bioscience · 2023Article
- Roles of retrovirus-derivedFrontiers in cell and developmental biology · 2023Review
- Allele-specific regulatory effects on the pig transcriptome.GigaScience · 2022Article
- Super-enhancers conserved within placental mammals maintain stem cell pluripotency.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Strategies to Improve the Efficiency of Somatic Cell Nuclear Transfer.International journal of molecular sciences · 2022Review
- Identification of the porcine IG-DMR and abnormal imprinting ofFrontiers in cell and developmental biology · 2022Article
- CBS and MAT2A improve methionine-mediated DNA synthesis through SAMTOR/mTORC1/S6K1/CAD pathway during embryo implantation.Cell proliferation · 2021Article
- Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How.International journal of molecular sciences · 2020Review
- MicroRNAs as Biomarkers in Canine Osteosarcoma: A New Future?Veterinary sciences · 2020Review
- No imprinted XIST expression in pigs: biallelic XIST expression in early embryos and random X inactivation in placentas.Cellular and molecular life sciences : CMLS · 2019Article
- Retrotransposon gag-like 1 (RTL1) and the molecular evolution of self-targeting imprinted microRNAs.Biology direct · 2019Article
- Various nuclear reprogramming systems using egg and oocyte materials.The Journal of reproduction and development · 2019Review
Corrections and comments
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Authors and funding
27 authors at 9 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Substantial rates of fetal loss plague all in vitro procedures involving embryo manipulations, including human-assisted reproduction, and are especially problematic for mammalian cloning where over 90% of reconstructed nuclear transfer embryos are typically lost during pregnancy. However, the epigenetic mechanism of these pregnancy failures has not been well described. Here we performed methylome and transcriptome analyses of pig induced pluripotent stem cells and associated cloned embryos, and revealed that aberrant silencing of imprinted genes, in particular the retrotransposon-derived RTL1 gene, is the principal epigenetic cause of pregnancy failure. Remarkably, restoration of RTL1 expression in pig induced pluripotent stem cells rescued fetal loss. Furthermore, in other mammals, including humans, low RTL1 levels appear to be the main epigenetic cause of pregnancy failure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.