Evidence map›Paper›PMID 30380914›Full record

ArticleCell transplantation2019

Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate in vivo and Promote Facial Nerve Regeneration.

Larissa Vilela Pereira, Ricardo Ferreira Bento, Dayane B Cruz, Cláudia Marchi, Raquel Salomone, Jeanne Oiticicca, Márcio Paulino Costa, Luciana A Haddad, Regina Célia Mingroni-Netto, Heloisa Juliana Zabeu Rossi Costa

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In one paragraph

Article in Cell transplantation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Larissa Vilela Pereira1 Department of Ophthalmology and Otolaryngology, University of Sao Paulo, School of Medicine, Brazil.ORCID 0000-0003-3284-5641
Ricardo Ferreira Bento1 Department of Ophthalmology and Otolaryngology, University of Sao Paulo, School of Medicine, Brazil.ORCID 0000-0003-3749-4684
Dayane B Cruz3 Human Genome and Stem-Cell Research Center, Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, Brazil.
Cláudia Marchi2 Medical Investigations Laboratory of Otolaryngology/LIM 32, University of São Paulo, Brazil.
Raquel Salomone1 Department of Ophthalmology and Otolaryngology, University of Sao Paulo, School of Medicine, Brazil.
Jeanne Oiticicca1 Department of Ophthalmology and Otolaryngology, University of Sao Paulo, School of Medicine, Brazil.
Márcio Paulino Costa4 Department of Plastic Surgery, University of São Paulo, School of Medicine, Brazil.
Luciana A Haddad3 Human Genome and Stem-Cell Research Center, Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, Brazil.
Regina Célia Mingroni-Netto3 Human Genome and Stem-Cell Research Center, Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, Brazil.
Heloisa Juliana Zabeu Rossi Costa1 Department of Ophthalmology and Otolaryngology, University of Sao Paulo, School of Medicine, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Post-traumatic lesions with transection of the facial nerve present limited functional outcome even after repair by gold-standard microsurgical techniques. Stem cell engraftment combined with surgical repair has been reported as a beneficial alternative. However, the best association between the source of stem cell and the nature of conduit, as well as the long-term postoperative cell viability are still matters of debate. We aimed to assess the functional and morphological effects of stem cells from human exfoliated deciduous teeth (SHED) in polyglycolic acid tube (PGAt) combined with autografting of rat facial nerve on repair after neurotmesis. The mandibular branch of rat facial nerve submitted to neurotmesis was repaired by autograft and PGAt filled with purified basement membrane matrix with or without SHED. Outcome variables were compound muscle action potential (CMAP) and axon morphometric. Animals from the SHED group had mean CMAP amplitudes and mean axonal diameters significantly higher than the control group ( p < 0.001). Mean axonal densities were significantly higher in the control group ( p = 0.004). The engrafted nerve segment resected 6 weeks after surgery presented cells of human origin that were positive for the Schwann cell marker (S100), indicating viability of transplanted SHED and a Schwann cell-like phenotype. We conclude that regeneration of the mandibular branch of the rat facial nerve was improved by SHED within PGAt. The stem cells integrated and remained viable in the neural tissue for 6 weeks since transplantation, and positive labeling for S100 Schwann-cell marker suggests cells initiated in vivo differentiation.

Indexed as

AnimalsAxonsFluorescent Antibody TechniqueHumansMaleMesenchymal Stem CellsNerve RegenerationRats, WistarStem CellsTooth, Deciduousautograftfacial nervefacial nerve regenerationhuman exfoliated deciduous teeth stem cell (SHED)nerve repairpolyglycolic acid tube

Identifiers

PMID30380914
PMCPMC6322138

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.