ArticlePloS one2018
Shotgun metagenomics of honey DNA: Evaluation of a methodological approach to describe a multi-kingdom honey bee derived environmental DNA signature.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Exploration of viral diversity in Aedes mosquitoes employing different shotgun metagenomic data analysis pipelines.Virology journal · 2026Article
- Review
- Honey environmental DNA reveals entomological fingerprints through dual mitochondrial cytochrome c oxidase subunit 1 (COI) and cytochrome b (CYTB) metabarcoding.Scientific reports · 2026Article
- Biogeography from a food matrix: a temporal distribution map of Apis mellifera mitochondrial DNA lineages across Italy, obtained from honey samples.Scientific reports · 2026Article
- The Evolution of Bees: Insights from 'Omic Studies.Genome biology and evolution · 2026Review
- Inferring plant-bee-microbe associations: Foragers, hive workers, and honey tell complementary stories.PloS one · 2026Article
- A pilot proof-of-concept study of microbial and botanical diversity in honey samples from Necochea, Argentina.Frontiers in microbiology · 2026Article
- Article
- Genome-resolved metagenomics reveals a phylogenetically cohesiveISME communications · 2026Article
- Metagenomic analysis of microbial communities and associated resistance genes, virulence genes, and mobile genetic elements in natural honey from Mu Cang Chai, Vietnam.World journal of microbiology & biotechnology · 2025Article
- Article
- Honey bulk DNA metagenomic analysis to identify honey biological composition and monitor honey bee pathogens.NPJ science of food · 2025Article
- The Latitudinal Biotic Interaction Hypothesis revisited: contrasting latitudinal richness gradients in actively vs. passively accumulated interaction partners of honey bees.BMC ecology and evolution · 2025Article
- DNA in honey could describe the changes in flower visits and microbe encounters of honey bees over decades.Scientific reports · 2025Article
- Characterization of Honey Microbiome Using MALDI-TOF Mass Spectrometry and Physicochemical Study.Molecules (Basel, Switzerland) · 2025Review
- Gut Microbiome Diversity in European Honeybees (Insects · 2025Article
- Article
- Honey microbiota, methods for determining the microbiological composition and the antimicrobial effect of honey - A review.Food chemistry: X · 2024Review
- Molecular Detection and Phylogenetic Relationships of Honey Bee-Associated Viruses in Bee Products.Veterinary sciences · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Honey bees are considered large-scale monitoring tools due to their environmental exploration and foraging activities. Traces of these activities can be recovered in the honey that also may reflect the hive ecological micro-conditions in which it has been produced. This study applied a next generation sequencing platform (Ion Torrent) for shotgun metagenomic analysis of honey environmental DNA (eDNA). The study tested a methodological framework to interpret DNA sequence information useful to describe the complex ecosystems of the honey bee colony superorganism, its pathosphere and the heterogeneity of the agroecological environments and environmental sources that left DNA marks in the honey. Analysis of two honeys reported sequence reads from five main organism groups (kingdoms or phyla): arthropods (that mainly included reads from Apis mellifera, several other members of the Hymenotpera, in addition to members of the Diptera, Coleoptera and Lepidoptera, as well as aphids and mites), plants (that clearly confirmed the botanical origin of the two honeys, i.e. orange tree blossom and eucalyptus tree blossom honeys), fungi and bacteria (including common hive and honey bee gut microorganisms, honey bee pathogens and plant pathogens), and viruses (which accounted for the largest number of reads in both honeys, mainly assigned to Apis mellifera filamentous virus). The shotgun metagenomic approach that was used in this study can be applied in large scale experiments that might have multiple objectives according to the multi-kingdom derived eDNA that is contained in the honey.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.