ArticlePloS one2018
Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
100 citing papers in PubMed, 218 citations in OpenAlex.
- ABCG transporters are involved in the accumulation of specialized metabolites in the oil bodies of Marchantia polymorpha.Plant & cell physiology · 2026Article
- Dexamethasone-inducible LhGR/pOp system: simple and flexible spatiotemporal control of gene expression.Planta · 2026Review
- A key role for S-nitrosylation in immune regulation and development in the liverwort Marchantia polymorpha.Journal of experimental botany · 2026Article
- Jasmonate Biosynthesis Across Bryophyte Lineages: Lessons fromPlants (Basel, Switzerland) · 2026Review
- Ancient peptide-redox signalling underlies sperm motility in Marchantia.Nature plants · 2026Article
- A simple cell-cycle control system in Marchantia polymorpha provides a framework for understanding plant cell proliferation.The Plant cell · 2026Article
- Conservation and divergence of UVR8 photoreceptor-mediated UV-B signaling in Marchantia polymorpha.Plant physiology · 2026Article
- SWI3A/B regulates the transition from vegetative to reproductive phase in the liverwort Marchantia polymorpha.Plant reproduction · 2026Article
- Initiation of asexual reproduction by the AP2/ERF gene GEMMIFER in Marchantia polymorpha.Current biology : CB · 2026Article
- MarpolBase: genome database for Marchantia polymorpha featuring high quality reference genome sequences.Plant & cell physiology · 2026Article
- Reversible phosphorylation of NPH3/RPT2-like proteins regulates phototropin receptor signaling.The Plant cell · 2026Article
- Spatial distribution of isoprenoid enzymes and MpABCG1 transporter influences sesquiterpene accumulation in Marchantia polymorpha oil bodies.Communications biology · 2026Article
- The SPL-family transcription factor MpSPL3 orchestrates the proper regulation of vegetative and reproductive programs in Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2026Article
- Amyloplasts are necessary for full gravitropism in thallus of Marchantia polymorpha.Journal of experimental botany · 2025Article
- The MpCAFA gene encodes a ciliary protein required for spermatozoid motility in the liverwort Marchantia polymorpha.Journal of plant research · 2025Article
- GA-independent DELLA regulation by inositol pyrophosphate in a nonvascular land plant.Nature chemical biology · 2025Article
- Conservation of heat stress acclimation by the IPK2-type kinases that control the synthesis of the inositol pyrophosphate 4/6-InsP7 in land plants.PLoS genetics · 2025Article
- SHOT GLASS, an R2R3-MYB transcription factor, promotes gemma cup and gametangiophore development in Marchantia polymorpha.The New phytologist · 2025Article
- MpmiR319 promotes gemma/gemma cup formation in the liverwort Marchantia polymorpha.Journal of experimental botany · 2025Article
- CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
40 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors with high efficiency for both construction and genome editing. Codon optimization of Cas9 to Arabidopsis achieved over 70% genome editing efficiency at two loci tested. Systematic assessment revealed that guide sequences of 17 nt or shorter dramatically decreased this efficiency. We also demonstrated that a combinatorial use of this system and a floxed complementation construct enabled conditional analysis of a nearly essential gene. This study reports that simple, rapid, and efficient genome editing is feasible with the series of developed vectors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.