ArticleCurrent protocols in cytometry2019
Multiphoton Imaging of Collagen, Elastin, and Calcification in Intact Soft-Tissue Samples.
Article in Current protocols in cytometry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Collagen-elastin dermal scaffolds enhance tissue regeneration and reduce scarring in preclinical models.Materials today. Bio · 2025Article
- Load Transfer along Continuous Collagen Fibers Reduces the Importance of Wall Thickness Variations.ArXiv · 2025Article
- MALDI mass spectrometry imaging of extracellular matrix proteins.The Analyst · 2025Review
- Nonlinear Imaging Detection of Organ Fibrosis in Minute Samples for Early Stage Utilizing Dual-Channel Two-Photon and Second-Harmonic Excitation.Biosensors · 2025Article
- Characterization of mechanical damage and viscoelasticity on aortas from guinea pigs subjected to hypoxia.Scientific reports · 2025Article
- Effect of melatonin on passive, ex-vivo biomechanical behavior of lamb esophagus.Scientific reports · 2025Article
- Endothelial dysfunction promotes age-related reorganization of collagen fibers and alters aortic biomechanics in mice.American journal of physiology. Heart and circulatory physiology · 2025Article
- Protective biomechanical and histological changes in the false lumen wall in chronic type B aortic dissection.JTCVS open · 2025Article
- Phasor-FLIM and SHG imaging for quantitative analysis of lung cancer autofluorescence.Computational and structural biotechnology journal · 2025Article
- Understanding the effects of mineralization and structure on the mechanical properties of tendon-bone insertion using mesoscale computational modeling.Journal of the mechanical behavior of biomedical materials · 2024Article
- Atherosclerotic Calcifications Have a Local Effect on the Peel Behavior of Human Aortic Media.Journal of biomechanical engineering · 2024Article
- A discrete fiber network finite element model of arterial elastin network considering inter-fiber crosslinking property and density.Journal of the mechanical behavior of biomedical materials · 2022Article
- Tissue compartmentalization enablesProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Effect of macro-calcification on the failure mechanics of intracranial aneurysmal wall tissue.Experimental mechanics · 2021Article
- Characterization of fibrillar collagen isoforms in infarcted mouse hearts using second harmonic generation imaging.Biomedical optics express · 2021Article
- Nonlinear optical microscopy is a novel tool for the analysis of cutaneous alterations in pseudoxanthoma elasticum.Lasers in medical science · 2020Article
- Adipose-derived stromal cell secreted factors induce the elastogenesis cascade within 3D aortic smooth muscle cell constructs.Matrix biology plus · 2019Article
- Calcification in Human Intracranial Aneurysms Is Highly Prevalent and Displays Both Atherosclerotic and Nonatherosclerotic Types.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Multiphoton-induced second-harmonic generation and two-photon excitation enable imaging of collagen and elastin fibers at micron-level resolution to depths of hundreds of microns, without the use of exogenous stains. These attributes can be leveraged for quantitative analysis of the 3D architecture of collagen and elastin fibers within intact, soft tissue specimens such as the artery and bladder wall. This architecture influences the function of intramural cells and also plays a primary role in determining tissue passive mechanical properties. Calcification deposition in soft tissues is a highly prevalent pathology in both older and diseased populations that can alter tissue properties. In this unit, we provide a protocol for simultaneous multiphoton microscopy (MPM) imaging and analysis of 3D collagen and elastin structures with calcification, which is effective for fixed and fresh intact samples. We also provide an associated micro-CT protocol to identify regions of interest in the samples as a means to target the MPM imaging. © 2018 by John Wiley & Sons, Inc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.