ArticleRevista do Instituto de Medicina Tropical de Sao Paulo2018
Assessment and comparison of bacterial load levels determined by quantitative amplifications in blood culture-positive and negative neonatal sepsis.
Article in Revista do Instituto de Medicina Tropical de Sao Paulo, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 3 of them syntheses that pooled it.
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Who cites it
23 citing papers in PubMed, 3 syntheses or guidelines pooled it, 36 citations in OpenAlex.
- Molecular assays for the diagnosis of sepsis in neonates: a diagnostic test accuracy review.The Cochrane database of systematic reviews · 2025Pooled it
- Shorter versus longer duration antibiotic regimens for treatment of suspected neonatal sepsis.The Cochrane database of systematic reviews · 2024Pooled it
- Contemporary Trends in Global Mortality of Sepsis Among Young Infants Less Than 90 Days: A Systematic Review and Meta-Analysis.Frontiers in pediatrics · 2022Pooled it
- Multiple cross displacement amplification coupled with a lateral flow biosensor for ultra-rapid and highly sensitive detection ofMicrobiology spectrum · 2026Article
- A comparison of routine blood culture methods and multiplex quantitative PCR for detecting pathogens in simulated polymicrobial blood cultures.Journal of medical microbiology · 2026Article
- Nanomotion-enabled ultra-rapid antibiotic susceptibility testing with magnetic bead-based pathogen enrichment for accelerated sepsis diagnostics.Scientific reports · 2026Article
- Dual RNA isolation from blood: an optimized protocol for host and bacterial RNA purification for dual RNA-sequencing analysis in whole blood sepsis samples.Microbial genomics · 2025Article
- Differential roles of interleukin-6 and adrenomedullin in early diagnosis and mortality predictions in late-onset neonatal sepsis.Clinical and experimental pediatrics · 2025Article
- Pediatric blood cultures-turning up the volume: a before and after intervention study.European journal of pediatrics · 2024Article
- Universal Digital High-Resolution Melt Analysis for the Diagnosis of Bacteremia.The Journal of molecular diagnostics : JMD · 2024Article
- Comparative Pharmacokinetics of Gentamicin CAntibiotics (Basel, Switzerland) · 2024Article
- Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses.Biomedicines · 2024Article
- Diagnostic status and epidemiological characteristics of community-acquired bacterial meningitis in children from 2019 to 2020: a multicenter retrospective study.BMC pediatrics · 2024Article
- Outer Membrane Vesicles (OMVs) as Biomedical Tools and Their Relevance as Immune-Modulating Agents againstInternational journal of molecular sciences · 2023Review
- Metagenomic Next-Generation Sequencing for the Diagnosis of Neonatal Infectious Diseases.Microbiology spectrum · 2022Article
- Impact of delayed processing of positive blood cultures on organism detection: a prospective multi-centre study.BMC infectious diseases · 2022Article
- Molecular Methodologies for Improved Polymicrobial Sepsis Diagnosis.International journal of molecular sciences · 2022Review
- Determining the Clinical Utility of 16S rRNA Sequencing in the Management of Culture-Negative Pediatric Infections.Antibiotics (Basel, Switzerland) · 2022Article
- Look Who's Talking: Host and Pathogen Drivers ofInternational journal of molecular sciences · 2022Review
- A pentaplex real-time PCR assay for rapid identification of major beta-lactamase genes KPC, NDM, CTX, CMY, and OXA-48 directly from bacteria in blood.Journal of medical microbiology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial sepsis remains a major cause of mortality and blood cultures are the gold standard of laboratory diagnosis even though they lack sensitivity in neonates. Culturenegative sepsis, also known as clinical sepsis, has long been considered a diagnosis in neonatal intensive care units because, as well as culture-positive infants, culture-negative neonates have worse prognosis in comparison with non-infected ones. Quantitative amplifications are used to detect bacterial infections in neonates but results are considered only in a qualitative way (positive or negative). The aim of the present study was to determine and compare bacterial load levels in blood culture-positive and culture-negative neonatal sepsis. Seventy neonates with clinical and laboratory evidence of infection admitted at three neonatal intensive care units were classified as blood culture-positive or culture-negative. Blood samples obtained at the same time of blood cultures had bacterial load levels assessed through a 16S rDNA qPCR. Blood cultures were positive in 29 cases (41.4%) and qPCR in 64 (91.4%). In the 29 culture-positive cases, 100% were also positive by qPCR, while in the 41 culture-negative cases, 35 (85.4%) were positive by qPCR. Bacterial load levels were in general < 50 CFU/mL, but were significantly higher in culture-positive cases (Mann-Whitney, p = 0.013), although clinical and laboratory findings were similar, excepting for deaths. In conclusion, the present study has shown that blood culture-negative neonates have lower bacteria load levels in their bloodstream when compared to blood culture-positive infants.
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