ArticleEcology and evolution2018
An environmental DNA sampling method for aye-ayes from their feeding traces.
Article in Ecology and evolution, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 37 citations in OpenAlex.
- Targeted sequencing enhances detection of pangolin trafficking hotspots and dynamics of both domestic and global trade markets.PLoS biology · 2026Article
- A hybrid genome assembly of the endangered aye-aye (Daubentonia madagascariensis).G3 (Bethesda, Md.) · 2024Article
- Nuclear environmental DNA resolves fine-scale population genetic structure in an aquatic habitat.iScience · 2024Article
- The predator problem and PCR primers in molecular dietary analysis: Swamped or silenced; depth or breadth?Molecular ecology resources · 2023Review
- Two hundred and five newly assembled mitogenomes provide mixed evidence for rivers as drivers of speciation for Amazonian primates.Molecular ecology · 2022Article
- Performance and automation of ancient DNA capture with RNA hyRAD probes.Molecular ecology resources · 2022Article
- Leveraging eDNA to expand the study of hybrid zones.Molecular ecology · 2020Article
- Review
- An environmental DNA sampling method for aye-ayes from their feeding traces.Ecology and evolution · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noninvasive sampling is an important development in population genetic monitoring of wild animals. Particularly, the collection of environmental DNA (eDNA) which can be collected without needing to encounter the target animal facilitates the genetic analysis of endangered species. One method that has been applied to these sample types is target capture and enrichment which overcomes the issue of high proportions of exogenous (nonhost) DNA from these lower quality samples. We tested whether target capture of mitochondrial DNA from sampled feeding traces of the aye-aye, an endangered lemur species would yield mitochondrial DNA sequences for population genetic monitoring. We sampled gnawed wood where aye-ayes excavate wood-boring insect larvae from trees. We designed RNA probes complementary to the aye-aye's mitochondrial genome and used these to isolate aye-aye DNA from other nontarget DNA in these samples. We successfully retrieved six near-complete mitochondrial genomes from two sites within the aye-aye's geographic range that had not been sampled previously. Our method demonstrates the application of next-generation molecular techniques to species of conservation concern. This method can likely be applied to alternative foraged remains to sample endangered species other than aye-ayes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.