ArticleNature methods2018
A high-throughput pipeline for validation of antibodies.
Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
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- Comparative evaluation of Olink Explore 3072 and mass spectrometry with peptide fractionation for plasma proteomics.Communications chemistry · 2025Article
- Mesenchymal stromal cells induce neutrophil aggregation and extracellular vesicle storms for systemic lupus erythematosus.Signal transduction and targeted therapy · 2025Article
- A consensus platform for antibody characterization.Nature protocols · 2025Article
- An intranasal subunit vaccine induces protective systemic and mucosal antibody immunity against respiratory viruses in mouse models.Nature communications · 2025Article
- Vaccine responses and hybrid immunity in people living with HIV after SARS-CoV-2 breakthrough infections.NPJ vaccines · 2024Article
- Optimal decision-making in high-throughput virtual screening pipelines.Patterns (New York, N.Y.) · 2023Article
- A T cell receptor targeting a recurrent driver mutation in FLT3 mediates elimination of primary human acute myeloid leukemia in vivo.Nature cancer · 2023Article
- Multiplexed selectivity screening of anti-GPCR antibodies.Science advances · 2023Article
- Optimal high-throughput virtual screening pipeline for efficient selection of redox-active organic materials.iScience · 2023Article
- Using proteins to test for COVID antibodies.Nature · 2022Article
- Immune responses in Omicron SARS-CoV-2 breakthrough infection in vaccinated adults.Nature communications · 2022Article
- Rituximab-treated patients with lymphoma develop strong CD8 T-cell responses following COVID-19 vaccination.British journal of haematology · 2022Article
- Target organ expression and biomarker characterization of chemokine CCL21 in systemic sclerosis associated pulmonary arterial hypertension.Frontiers in immunology · 2022Article
- Deepening into Intracellular Signaling Landscape through Integrative Spatial Proteomics and Transcriptomics in a Lymphoma Model.Biomolecules · 2021Article
- Opinion: Independent third-party entities as a model for validation of commercial antibodies.New biotechnology · 2021Article
- A ChIP-exo screen of 887 Protein Capture Reagents Program transcription factor antibodies in human cells.Genome research · 2021Article
- Towards reproducibility in large-scale analysis of protein-protein interactions.Nature methods · 2021Article
- Systemic complement activation is associated with respiratory failure in COVID-19 hospitalized patients.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Resistance training increases muscle NADAging · 2020Article
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.