ArticleNature communications2018
Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed.
- Mfsd2a is important for maintaining epidermal homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- From basic biology to engineered therapies: the keratinocyte stem cell playbook.Frontiers in medical technology · 2026Review
- Retina-specific laminin-based generation of photoreceptor progenitors from human pluripotent stem cells under xeno-free and chemically defined conditions.Nature protocols · 2025Review
- Towards propagation of epidermal cells for wound repair: glass, as cell culture substrate, enhances proliferation and migration of human keratinocytes.Frontiers in bioengineering and biotechnology · 2025Article
- Guided monocyte fate to FRβ/CD163Cellular and molecular life sciences : CMLS · 2024Article
- Article
- Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.NPJ Regenerative medicine · 2023Article
- Photoreceptor laminin drives differentiation of human pluripotent stem cells to photoreceptor progenitors that partially restore retina function.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Corneal epithelial basement membrane assembly is mediated by epithelial cells in coordination with corneal fibroblasts during wound healing.Molecular vision · 2023Article
- Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model.International journal of molecular sciences · 2022Article
- Embigin is a fibronectin receptor that affects sebaceous gland differentiation and metabolism.Developmental cell · 2022Article
- Optimizing large-scale autologous human keratinocyte sheets for major burns-Toward an animal-free production and a more accessible clinical application.Health science reports · 2022Article
- Laminin-511-derived recombinant fragment and Rho kinase inhibitor Y-27632 facilitate serial cultivation of keratinocytes differentiated from human embryonic stem cells.Regenerative therapy · 2021Article
- Tissue-specific parameters for the design of ECM-mimetic biomaterials.Acta biomaterialia · 2021Review
- Human primary epidermal organoids enable modeling of dermatophyte infections.Cell death & disease · 2021Article
- Culture of human ovarian tissue in xeno-free conditions using laminin components of the human ovarian extracellular matrix.Journal of assisted reproduction and genetics · 2020Article
- Decrease of laminin-511 in the basement membrane due to photoaging reduces epidermal stem/progenitor cells.Scientific reports · 2020Article
- Chemically defined and xenogeneic-free culture method for human epidermal keratinocytes on laminin-based matrices.Nature protocols · 2020Article
- Automated collective motion analysis validates human keratinocyte stem cell cultures.Scientific reports · 2019Article
- Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood-brain barrier model.Fluids and barriers of the CNS · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
The current expansion of autologous human keratinocytes to resurface severe wound defects still relies on murine feeder layer and calf serum in the cell culture system. Through our characterization efforts of the human skin basement membrane and murine feeder layer 3T3-J2, we identified two biologically relevant recombinant laminins-LN-511 and LN-421- as potential candidates to replace the murine feeder. Herein, we report a completely xeno-free and defined culture system utilizing these laminins which enables robust expansion of adult human skin keratinocytes. We demonstrate that our laminin system is comparable to the 3T3-J2 co-culture system in terms of basal markers' profile, colony-forming efficiency and the ability to form normal stratified epidermal structure in both in vitro and in vivo models. These results show that the proposed system may not only provide safer keratinocyte use in the clinics, but also facilitate the broader use of other cultured human epithelial cells in regenerative medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.