Evidence map›Paper›PMID 30377127›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2018

[Rapid preimplantation genetic diagnosis of

Huiling Xu, Yanhui Liu, Ping Yan, Yi He, Jiachun Qin, Jiwu Lou, Wanjun Zhou

Abstract read
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 84% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Huiling XuDepartment of Medical Genetics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
Yanhui LiuDongguan Maternal and Children's Healthcare Hospital, Dongguan 523122, China.
Ping YanHuiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Yi HeDongguan Maternal and Children's Healthcare Hospital, Dongguan 523122, China.
Jiachun QinDepartment of Medical Genetics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
Jiwu LouDongguan Maternal and Children's Healthcare Hospital, Dongguan 523122, China.
Wanjun ZhouDepartment of Medical Genetics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
Dongguan People’s Hospital · CNSouthern Medical University · CNNanfang Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo develop a rapid preimplantation genetic diagnosis method for

methodsUsing multiple displacement amplification (MDA) WGA technique, we established a double-fluorescent PCR system of the housekeeping genes GAPDH and β-actin for WGA quality testing, and a genotyping PCR system of mutant and normal short sequences for α-thalassemia SEA deletion. The sensitivity and accuracy of this method for diagnosis of

resultsDetection of lymphocyte samples with different cell numbers using the method developed in this study revealed no ADO in 3-cell samples, and the product quantity of WGA became stable for 4-cell samples. Genotyping of the 10 blastocyst biopsy samples with successful WGA showed a genotype of --

conclusionsThe method developed in this study is a complete testing process for 4-6 blastocyst biopsy cells to allow rapid, accurate, and cost-effective PGD genotyping of

Indexed as

BlastocystGene Deletionalpha-ThalassemiaAsia, SoutheasternFemaleGenome-Wide Association StudyHumansPolymerase Chain ReactionPregnancyPreimplantation DiagnosisSensitivity and SpecificitySequence DeletiongenotypingMDAPGDα-Thalassemia

Identifiers

PMID30377127
PMCPMC6744053
OpenAlexW2903471307

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.