Evidence map›Paper›PMID 30377111›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2018

[Role of miR-144-3p and its target gene in regulating osteogenic differentiation of rat bone marrow mesenchymal stem cells

Jin Lu, Haoxuan Zhang, Peng Yu, Yifeng Gong, Xiwang Gong, Qiangqiang Fan, Yue Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. SMAD4 contributes to chondrocyte and osteocyte development.Journal of cellular and molecular medicine · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jin LuKey Laboratory of Organization and Transplantation of Anhui Province, Bengbu 233000, China.
Haoxuan ZhangDepartment of Human Anatomy, Bengbu Medical College, Bengbu 233030, China.
Peng YuDepartment of Human Anatomy, Bengbu Medical College, Bengbu 233030, China.
Yifeng GongDepartment of Obstetrics and Gynecology, Wuhu Fanchang Hospital of Traditional Chinese Medicine, Wuhu 241200, China.
Xiwang GongDepartment of Obstetrics and Gynecology, Wuhu Fanchang Hospital of Traditional Chinese Medicine, Wuhu 241200, China.
Qiangqiang FanShanghai Institute of life Science, Chinese Academy of Sciences, Shanghai 200233, China.
Yue YangDepartment of Obstetrics and Gynecology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China.
Bengbu Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the role of miR-144-3p in regulating osteogenic differentiation of bone marrow mesenchymal stem cells and predict its target genes.

methodsRat bone marrow mesenchymal stem cells (BMSCs) with induced osteogenic differentiation were examined for the expressions of Runx2, OCN and miR-144-3p. The effects of transfection with a miR-144-3p mimic or a miR-144-3p inhibitor were tested on the osteogenic differentiation of the BMSCs. The changes in the expressions of the predicted target of miR-144-3p in the BMSCs during induced osteogenic differentiation were examined using Western blotting and qRT-PCR.

resultsRat BMSCs with induced differentiation into osteoblasts exhibited a progressive increase in the expressions of Runx2 and OCN (two markers of osteogenic differentiation), while the expression of miR-144-3p gradually decreased during the differentiation till reaching the lowest level at 21 days of induction. In rat BMSCs, transfection with the miR-144-3p mimic significantly decreased ALP activity (

conclusionsmiR-144-3p participates in the regulation of osteogenic differentiation of rat BMSCs, and its inhibitory effect on osteogenic differentiation is achieved probably by decreasing the expression level of Smad4.

Indexed as

Cell DifferentiationAnimalsBiomarkersBone Marrow CellsCalcium-Binding ProteinsCells, CulturedCore Binding Factor Alpha 1 SubunitMesenchymal Stem CellsMicroRNAsOsteoblastsOsteogenesisRatsSmad4 ProteinTransfectionBiomarkersCalcium-Binding ProteinsCore Binding Factor Alpha 1 SubunitMicroRNAsMIRN144 microRNA, ratoncomodulinRunx2 protein, ratSmad4 ProteinSmad4 protein, ratbone marrow mesenchymal stem cellsmiR-144-3posteogenic differentiation

Identifiers

PMID30377111
PMCPMC6744198
OpenAlexW2897161053

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.