ArticleVirology journal2018
A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus.
Article in Virology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- RAZOR: a database of PCR primers targeting human respiratory viruses.NAR genomics and bioinformatics · 2026Article
- Respiratory Infection-Related Pathogens in the Pediatric Intensive Care Unit During 2019-2024 in Hubei, China.Pathogens (Basel, Switzerland) · 2026Article
- Article
- Human metapneumovirus: pathogenesis, epidemiology, diagnostic technologies, and potential intervention strategies.Virology journal · 2025Review
- Review
- Detection of Rhinovirus in Clinical Samples by PCR.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Epidemiology and diagnosis technologies of human metapneumovirus in China: a mini review.Virology journal · 2024Review
- Reference material development for detection of human respiratory syncytial virus using digital PCR.Analytical and bioanalytical chemistry · 2023Article
- Development of a diagnostic assay by three-tube multiplex real-time PCR for simultaneous detection of nine microorganisms causing acute respiratory infections.Scientific reports · 2022Article
- Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications.The Analyst · 2021Review
- Evaluation of one-step RT-PCR multiplex assay for body fluid identification.International journal of legal medicine · 2021Article
- Comparison of three TaqMan real-time reverse transcription-PCR assays in detecting SARS-CoV-2.Journal of virological methods · 2021Article
- RAP: A Novel Approach to the Rapid and Highly Sensitive Detection of Respiratory Viruses.Frontiers in bioengineering and biotechnology · 2021Review
- Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2.Virology journal · 2020Article
- Recent advances in the detection of respiratory virus infection in humans.Journal of medical virology · 2020Review
- A novel real-time PCR assay panel for detection of common respiratory pathogens in a convenient, strip-tube array format.Journal of virological methods · 2019Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundRespiratory syncytial virus (RSV), human Rhinovirus (HRV) and human Metapneumo Virus (HMPV) are important viral pathogens causing acute respiratory tract infections in the hospitalized patients. Sensitive and accurate detection of RSV, HRV and HMPV is necessary for clinical diagnosis and treatment.
resultsA locked nucleic acid (LNA)-based multiplex closed one-tube nested real-time RT-PCR (mOTNRT-PCR) assay was developed for simultaneous detection of RSV, HRV and HMPV. The sensitivity, specificity, reproducibility and clinical performance of mOTNRT-PCR were evaluated and compared with individual real time PCR (RT-qPCR) assay using clinical samples. The analytical sensitivity of mOTNRT-PCR assay was 5 copies/reaction for RSV, HRV and HMPV, respectively, and no cross-reaction with other common respiratory viruses was observed. The coefficients of variation (CV) of intra-assay and inter-assay were between 0.51 to 3.67%. Of 398 nasopharyngeal aspirates samples tested, 109 (27.39%), 150 (37.69%) and 44 (11.06%) were positive for RSV, HRV and HMPV, respectively, whereas 95 (23.87%), 137 (34.42%) and 38 (9.55%) were positive for RSV, HRV and HMPV, respectively, by individual RT-qPCR assay. Thirty three samples that were positive by mOTNRT-PCR but negative by RT-qPCR were confirmed as true positives by sequencing using reported traditional two-step nested PCR assay.
conclusionmOTNRT-PCR assay reveals extremely higher sensitivity than that of RT-qPCR assay for detecting RSV, HRV and HMPV in clinical settings.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.