ArticleBMC immunology2018
Blood handling and leukocyte isolation methods impact the global transcriptome of immune cells.
Article in BMC immunology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Intracerebral Hemorrhage Induces Monocyte TNF Signaling in Patients That Is Suppressed by BAF312.Annals of clinical and translational neurology · 2026Article
- Single-cell analysis of microglia and monocyte dynamics uncovers distinct TNF-driven neuroimmune signatures in humans after intracerebral hemorrhage.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026Article
- Single-Cell Analysis of Microglia and Monocyte Dynamics Uncover Distinct TNF-α-driven Neuroimmune Signatures after Intracerebral Hemorrhage.bioRxiv : the preprint server for biology · 2026Article
- Intracerebral hemorrhage induces monocyte TNF signaling that is suppressed by Siponimod (BAF312): a single-cell transcriptomics study in patients.medRxiv : the preprint server for health sciences · 2026Article
- Follicle-intrinsic and spatially distinct molecular programs drive follicle rupture and luteinization during ex vivo mammalian ovulation.Communications biology · 2024Article
- A Human Whole Blood Culture System Reveals Detailed Cytokine Release Profiles of Implant Materials.Medical devices (Auckland, N.Z.) · 2024Article
- Exercise-induced changes in gene expression do not mediate post exertional malaise in Gulf War illness.Brain, behavior, & immunity - health · 2023Article
- Development of an RNase H2 Activity Assay for Clinical Screening.Journal of clinical medicine · 2023Article
- The transcriptomics profiling of blood CD4 and CD8 T-cells in narcolepsy type I.Frontiers in immunology · 2023Article
- Circular RNAs in peripheral blood mononuclear cells are more stable than linear RNAs upon sample processing delay.Journal of cellular and molecular medicine · 2022Article
- Cellular and transcriptional diversity over the course of human lactation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Multimodal analysis for humaniScience · 2021Article
- Leukocyte dynamics after intracerebral hemorrhage in a living patient reveal rapid adaptations to tissue milieu.JCI insight · 2021Article
- Longitudinal transcriptomics define the stages of myeloid activation in the living human brain after intracerebral hemorrhage.Science immunology · 2021Article
- Intracerebral Hemorrhage Induces Inflammatory Gene Expression in Peripheral Blood: Global Transcriptional Profiling in Intracerebral Hemorrhage Patients.DNA and cell biology · 2019Article
- Correction to: blood handling and leukocyte isolation methods impact the global transcriptome of immune cells.BMC immunology · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundTranscriptional profiling with ultra-low input methods can yield valuable insights into disease, particularly when applied to the study of immune cells using RNA-sequencing. The advent of these methods has allowed for their use in profiling cells collected in clinical trials and other studies that involve the coordination of human-derived material. To date, few studies have sought to quantify what effects that collection and handling of this material can have on resulting data.
resultsWe characterized the global effects of blood handling, methods for leukocyte isolation, and preservation media on low numbers of immune cells isolated from blood. We found overall that storage/shipping temperature of blood prior to leukocyte isolation and sorting led to global changes in both CD8
conclusionsOur data underscore the necessity of processing samples with comparably defined protocols prior to transcriptional profiling and demonstrate that a filtration method can be applied to quickly isolate immune cells of interest while minimizing transcriptional bias.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.