ArticleScientific reports2018
Acid bone lysate activates TGFβ signalling in human oral fibroblasts.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed.
- Antibiofilm Activity of a Thermoformable Material Loaded With Essential Oil Solution.Clinical and experimental dental research · 2026Article
- A Functional TGF-β/Smad Assay for Targeted Profiling of Demineralized Bone Matrix-Derived Allograft Bioactivity.Journal of functional biomaterials · 2026Article
- Review
- Bone Allograft Acid Lysates Change the Genetic Signature of Gingival Fibroblasts.International journal of molecular sciences · 2023Article
- Gingival Fibroblasts Are Sensitive to Oral Cell Lysates Indicated by Their IL11 Expression.Bioengineering (Basel, Switzerland) · 2023Article
- In vitro activity of hyaluronic acid and human serum on periodontal biofilm and periodontal ligament fibroblasts.Clinical oral investigations · 2023Article
- Bioactivation of 3D Cell-Imprinted Polydimethylsiloxane Surfaces by Bone Protein Nanocoating for Bone Tissue Engineering.ACS omega · 2022Article
- RNAseq of TGF-β receptor type I kinase-dependent genes in oral fibroblast exposed to milk.BMC oral health · 2021Article
- Platelet-Rich Fibrin Increases BMP2 Expression in Oral Fibroblasts via Activation of TGF-β Signaling.International journal of molecular sciences · 2021Article
- Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling.Scientific reports · 2021Article
- Acid bone lysates reduce bone regeneration in rat calvaria defects.Journal of biomedical materials research. Part A · 2021Article
- Article
- Micellar Casein and Whey Powder Hold a TGF-β Activity and Regulate ID Genes In Vitro.Molecules (Basel, Switzerland) · 2021Article
- TGF-β Activity of a Demineralized Bone Matrix.International journal of molecular sciences · 2021Article
- Cleaning Teeth Reduces the Inflammatory Response of Macrophages to Acid Dentine Lysate.International journal of molecular sciences · 2020Article
- Proteomic Analysis of Porcine-Derived Collagen Membrane and Matrix.Materials (Basel, Switzerland) · 2020Article
- Relative Centrifugal Force (RCF;International journal of molecular sciences · 2020Article
- YBEY is an essential biogenesis factor for mitochondrial ribosomes.Nucleic acids research · 2020Article
- TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays.International journal of molecular sciences · 2020Article
- Liquid Platelet-Rich Fibrin and Heat-Coagulated Albumin Gel: Bioassays for TGF-β Activity.Materials (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Demineralized bone matrix is a widely used allograft from which not only the inorganic mineral but also embedded growth factors are removed by hydrochloric acid (HCl). The cellular response to the growth factors released during the preparation of demineralized bone matrix, however, has not been studied. Here we investigated the in vitro impact of acid bone lysate (ABL) prepared from porcine cortical bone chips on oral fibroblasts. Proteomic analysis of ABL revealed a large spectrum of bone-derived proteins including TGF-β1. Whole genome microarrays and RT-PCR together with the pharmacologic blocking of TGF-β receptor type I kinase with SB431542 showed that ABL activates the TGF-β target genes interleukin 11, proteoglycan 4, and NADPH oxidase 4. Interleukin 11 expression was confirmed at the protein level by ELISA. Immunofluorescence and Western blot showed the nuclear localization of Smad2/3 and increased phosphorylation of Smad3 with ABL, respectively. This effect was independent of whether ABL was prepared from mandible, calvaria or tibia. These results demonstrate that TGF-β is a major growth factor that is removed upon the preparation of demineralized bone matrix.
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