ArticleScientific reports2018
In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Para-uterine imaging protocol: Stabilizing living mouse embryos within the maternal abdomen for in vivo imaging and visually guided physiology.STAR protocols · 2026Article
- Choroid plexus apocrine secretion shapes CSF proteome during mouse brain development.Nature neuroscience · 2025Article
- Network state transitions during cortical development.Nature reviews. Neuroscience · 2024Review
- Lithium and its effects: does dose matter?International journal of bipolar disorders · 2024Review
- Calcium Signaling during Cortical Apical Dendrite Initiation: A Role for Cajal-Retzius Neurons.International journal of molecular sciences · 2023Article
- Cortical activity emerges in region-specific patterns during early brain development.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Cortical activity emerges in region-specific patterns during early brain development.bioRxiv : the preprint server for biology · 2023Article
- Calcium and activity-dependent signaling in the developing cerebral cortex.Development (Cambridge, England) · 2022Review
- Neurophysiology of the Developing Cerebral Cortex: What We Have Learned and What We Need to Know.Frontiers in cellular neuroscience · 2021Review
- Ketamine and Calcium Signaling-A Crosstalk for Neuronal Physiology and Pathology.International journal of molecular sciences · 2020Review
- Two-photon microscopic observation of cell-production dynamics in the developing mammalian neocortex in utero.Development, growth & differentiation · 2020Article
- How Do Electric Fields Coordinate Neuronal Migration and Maturation in the Developing Cortex?Frontiers in cell and developmental biology · 2020Review
- Early Actions of Neurotransmitters During Cortex Development and Maturation of Reprogrammed Neurons.Frontiers in synaptic neuroscience · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
Abstract
Prior to sensory experience spontaneous activity appears to play a fundamental role in the correct formation of prominent functional features of different cortical regions. The use of anaesthesia during pregnancy such as ketamine is largely considered to negatively affect neuronal development by interfering with synaptic transmission. Interestingly, the characteristics of spontaneous activity as well as the acute functional effects of maternal anaesthesia remain largely untested in the embryonic cortex in vivo. In the present work, we performed in vivo imaging of spontaneous calcium activity and cell motility in the marginal zone of the cortex of E14-15 embryos connected to the mother. We made use of a preparation where the blood circulation from the mother through the umbilical cord is preserved and fluctuations in intracellular calcium in the embryonic frontal cortex are acquired using two-photon imaging. We found that spontaneous transients were either sporadic or correlated in clusters of neuronal ensembles at this age. These events were not sensitive to maternal isoflurane anaesthesia but were strongly inhibited by acute in situ or maternal application of low concentration of the anaesthetic ketamine (a non-competitive antagonist of NMDA receptors). Moreover, simultaneous imaging of cell motility revealed a correlated strong sensitivity to ketamine. These results show that anaesthetic compounds can differ significantly in their impact on spontaneous early cortical activity as well as motility of cells in the marginal zone. The effects found in this study may be relevant in the etiology of heightened vulnerability to cerebral dysfunction associated with the use of ketamine during pregnancy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.