ArticleScientific reports2018
Intravital imaging with two-photon microscopy reveals cellular dynamics in the ischeamia-reperfused rat heart.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Real-Time Axial Motion Compensation for Intravital Two-Photon Imaging of Mechanically Loaded Bone.bioRxiv : the preprint server for biology · 2026Article
- NIR Excitation in Atomically Precise Nanoclusters via Two-Photon and Three-Photon Absorption.Accounts of chemical research · 2026Article
- Mapping of blood flow and slow speckle tissue dynamics using laser speckle contrast imaging.Biomedical optics express · 2025Article
- Sending the Signal to Bone: How Tumor-Derived EVs Orchestrate Pre-Metastatic Niche Formation and Skeletal Colonization.Biomedicines · 2025Review
- Strong Enhancement of Two-Photon Absorption and Emergence of Unusual Extinction Saturation in Silver Sulfide Quantum Dots Integrated with Gold and Silica Nanostructures.ACS applied materials & interfaces · 2025Article
- Pericytes in mouse heart.Frontiers in physiology · 2025Article
- Two-Photon Intravital Microscopy of Glioblastoma in a Murine Model.Journal of visualized experiments : JoVE · 2024Article
- A historical review of experimental imaging of the beating heart coronary microcirculation in vivo.Journal of anatomy · 2023Review
- Advances and challenges in intravital imaging of craniofacial and dental progenitor cells.Genesis (New York, N.Y. : 2000) · 2022Review
- Visualization of regenerating and repairing hearts.Clinical science (London, England : 1979) · 2022Article
- Immune cells in cardiac homeostasis and disease: emerging insights from novel technologies.European heart journal · 2022Review
- Revealing the nanometric structural changes in myocardial infarction models by time-lapse intravital imaging.Frontiers in bioengineering and biotechnology · 2022Article
- Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging.Scientific reports · 2021Article
- The frontier of live tissue imaging across space and time.Cell stem cell · 2021Review
- Multi-Scale Imaging of Vascular Pathologies in Cardiovascular Disease.Frontiers in medicine · 2021Review
- Label-Free Multiphoton Microscopy for the Detection and Monitoring of Calcific Aortic Valve Disease.Frontiers in cardiovascular medicine · 2021Review
- Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Probing the subcellular nanostructure of engineered human cardiomyocytes in 3D tissue.Microsystems & nanoengineering · 2021Article
- Evaluation of Nanoparticle Penetration in the Tumor Spheroid Using Two-Photon Microscopy.Biomedicines · 2020Article
- Label-free optical biomarkers detect early calcific aortic valve disease in a wild-type mouse model.BMC cardiovascular disorders · 2020Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in intravital microscopy have provided insight into dynamic biological events at the cellular level in both healthy and pathological tissue. However, real-time in vivo cellular imaging of the beating heart has not been fully established, mainly due to the difficulty of obtaining clear images through cycles of cardiac and respiratory motion. Here we report the successful recording of clear in vivo moving images of the beating rat heart by two-photon microscopy facilitated by cardiothoracic surgery and a novel cardiac stabiliser. Subcellular dynamics of the major cardiac components including the myocardium and its subcellular structures (i.e., nuclei and myofibrils) and mitochondrial distribution in cardiac myocytes were visualised for 4-5 h in green fluorescent protein-expressing transgenic Lewis rats at 15 frames/s. We also observed ischaemia/reperfusion (I/R) injury-induced suppression of the contraction/relaxation cycle and the consequent increase in cell permeability and leukocyte accumulation in cardiac tissue. I/R injury was induced in other transgenic mouse lines to further clarify the biological events in cardiac tissue. This imaging system can serve as an alternative modality for real time monitoring in animal models and cardiological drug screening, and can contribute to the development of more effective treatments for cardiac diseases.
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