ArticleYeast (Chichester, England)2019
A fast and tuneable auxin-inducible degron for depletion of target proteins in budding yeast.
Article in Yeast (Chichester, England), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- ABA-AA: A simple, reversible, and non-toxic anchor-away system for effective nuclear protein depletion.Cell reports methods · 2026Article
- Vacuolar transport and function of Saccharomyces cerevisiae sterol ester hydrolase Tgl1.FEBS letters · 2026Article
- Transcriptome-wide mRNP condensation precedes stress granule formation and excludes new mRNAs.Molecular cell · 2025Article
- Transcriptome-wide mRNP condensation precedes stress granule formation and excludes new mRNAs.bioRxiv : the preprint server for biology · 2025Article
- Article
- DNA lesions can frequently precede DNA:RNA hybrid accumulation.Nature communications · 2025Article
- Rapid depletion of target proteins in plants by an inducible protein degradation system.The Plant cell · 2024Article
- Selected humanization of yeast U1 snRNP leads to global suppression of pre-mRNA splicing and mitochondrial dysfunction in the budding yeast.RNA (New York, N.Y.) · 2024Article
- Rapid, efficient auxin-inducible protein degradation inmSphere · 2023Article
- Rapid, efficient auxin-inducible protein degradation inbioRxiv : the preprint server for biology · 2023Article
- Conditional protein degradation inFrontiers in bioengineering and biotechnology · 2023Article
- The mIAA7 degron improves auxin-mediated degradation in Caenorhabditiselegans.G3 (Bethesda, Md.) · 2022Article
- On the trail of auxin: Reporters and sensors.The Plant cell · 2022Review
- Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons.eLife · 2022Article
- Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast.RNA (New York, N.Y.) · 2022Article
- Controlling CRISPR with small molecule regulation for somatic cell genome editing.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Review
- Conditional depletion of transcriptional kinases Ctk1 and Bur1 and effects on co-transcriptional spliceosome assembly and pre-mRNA splicing.RNA biology · 2021Article
- Auxin-mediated protein depletion for metabolic engineering in terpene-producing yeast.Nature communications · 2021Article
- Article
- An improved auxin-inducible degron system preserves native protein levels and enables rapid and specific protein depletion.Genes & development · 2019Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The auxin-inducible degron (AID) is a useful technique to rapidly deplete proteins of interest in nonplant eukaryotes. Depletion is achieved by addition of the plant hormone auxin to the cell culture, which allows the auxin-binding receptor, TIR1, to target the AID-tagged protein for degradation by the proteasome. Fast depletion of the target protein requires good expression of TIR1 protein, but as we show here, high levels of TIR1 may cause uncontrolled depletion of the target protein in the absence of auxin. To enable conditional expression of TIR1 to a high level when required, we regulated the expression of TIR1 using the β-estradiol expression system. This is a fast-acting gene induction system that does not cause secondary effects on yeast cell metabolism. We demonstrate that combining the AID and β-estradiol systems results in a tightly controlled and fast auxin-induced depletion of nuclear target proteins. Moreover, we show that depletion rate can be tuned by modulating the duration of β-estradiol preincubation. We conclude that TIR1 protein is a rate-limiting factor for target protein depletion in yeast, and we provide new tools that allow tightly controlled, tuneable, and efficient depletion of essential proteins whereas minimising secondary effects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.