Evidence map›Paper›PMID 30374565›Full record

ArticleInternational journal of legal medicine2019

Massively parallel sequencing analysis of nondegraded and degraded DNA mixtures using the ForenSeq™ system in combination with EuroForMix software.

Hsiao-Lin Hwa, Ming-Yih Wu, Wan-Chia Chung, Tsang-Ming Ko, Chih-Peng Lin, Hsiang-I Yin, Tsui-Ting Lee, James Chun-I Lee

Abstract read
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Article in International journal of legal medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hsiao-Lin HwaDepartment and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen Ai Rd., Taipei, 100, Taiwan.
Ming-Yih WuDepartment of Obstetrics and Gynecology, National Taiwan University Hospital, No. 7 Chung Shan S. Rd., Taipei, 100, Taiwan.
Wan-Chia ChungYourgene Bioscience, No.376-5 Fuxing Rd., Shulin Dist., New Taipei City, 238, Taiwan.
Tsang-Ming KoGenephile Bioscience Laboratory, No. 10, 1 F, Lin Sen South Road, Zhong Zheng District, Taipei, 100, Taiwan.
Chih-Peng LinYourgene Bioscience, No.376-5 Fuxing Rd., Shulin Dist., New Taipei City, 238, Taiwan.
Hsiang-I YinDepartment and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen Ai Rd., Taipei, 100, Taiwan.
Tsui-Ting LeeDepartment and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen Ai Rd., Taipei, 100, Taiwan.
James Chun-I LeeDepartment and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen Ai Rd., Taipei, 100, Taiwan. jimlee@ntu.edu.tw.

Funding

Ministry of Science and Technology, Taiwan, R.O.C. MOST 103-2320-B-002-023-MY2National Taiwan University Hospital NTUH103-S2398
6 · The paper itself

Abstract

Massively parallel sequencing (MPS) technologies enable the simultaneous analysis of short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs). MPS also enables the detection of alleles of the minor contributors in imbalanced DNA mixtures. In this study, 59 STRs (amelogenin, 27 autosomal STRs, 7 X-STRs, and 24 Y-STRs) and 94 identity-informative SNPs of 119 unrelated Taiwanese (50 men, 69 women) were sequenced using a commercial MPS kit. Forty-eight nondegraded and 44 highly degraded two-person artificial DNA mixtures with various minor to major ratios (1:9, 1:19, 1:29, 1:39, 1:79, and 1:99) were analyzed to examine the performance of this system for detecting the alleles of the minor contributors in DNA mixtures. Likelihood ratios based on continuous model were calculated using the EuroForMix for DNA mixture interpretation. The STR and SNP genotypes of these 119 Taiwanese were obtained. Several sequence variants of STRs were observed. Using EuroForMix software based on the sequence data of autosomal STRs and autosomal SNPs, 97.9% (47/48) and 97.7% (42/43) of minor donors were accurately inferred among the successfully analyzed nondegraded and degraded DNA mixtures, respectively. In conclusion, combined with EuroForMix software, this commercial kit is effective for assignment of the minor contributors in nondegraded and degraded DNA mixtures.

Indexed as

DNA Degradation, NecroticSoftwareAsian PeopleDNA FingerprintingFemaleGene FrequencyGenotypeHigh-Throughput Nucleotide SequencingHumansLikelihood FunctionsMaleMicrosatellite RepeatsPolymorphism, Single NucleotideSequence Analysis, DNADNA mixtureEuroForMixForenSeq™ systemMassively parallel sequencingShort tandem repeatSingle nucleotide polymorphism

Identifiers

PMID30374565

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.