ArticleJournal of cell science2018
Roles for Ena/VASP proteins in FMNL3-mediated filopodial assembly.
Article in Journal of cell science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- CCT8 associates with the MYO10 motor domain and regulates filopodia and breast cancer cell invasion.Journal of cell science · 2026Article
- Development of ARL4C antisense oligonucleotide with reduced off-target effects and enhanced efficacy as an anti-cancer drug.In vitro cellular & developmental biology. Animal · 2026Article
- Spatially distinct FRL and Ena dependent actin networks coordinate nuclear positioning in Drosophila nurse cells.PLoS genetics · 2026Article
- The PTTG1/VASP axis promotes oral squamous cell carcinoma metastasis by modulating focal adhesion and actin filaments.Molecular oncology · 2025Article
- Upregulation of ENAH by a PI3K/AKT/β-catenin cascade promotes oral cancer cell migration and growth via an ITGB5/Src axis.Cellular & molecular biology letters · 2024Article
- An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis.Nature communications · 2024Article
- filoVision - using deep learning and tip markers to automate filopodia analysis.Journal of cell science · 2024Article
- Review
- The S2 Subunit of Infectious Bronchitis Virus Affects Abl2-Mediated Syncytium Formation.Viruses · 2023Article
- Spatiotemporal Regulation of FMNL2 by N-Terminal Myristoylation and C-Terminal Phosphorylation Drives Rapid Filopodia Formation.Biomolecules · 2023Article
- Activated I-BAR IRSp53 clustering controls the formation of VASP-actin-based membrane protrusions.Science advances · 2022Article
- Parallel kinase pathways stimulate actin polymerization at depolarized mitochondria.Current biology : CB · 2022Article
- Neisseria gonorrhoeae subverts formin-dependent actin polymerization to colonize human macrophages.PLoS pathogens · 2021Article
- Myosin-X and talin modulate integrin activity at filopodia tips.Cell reports · 2021Article
- Article
- Intra-bundle contractions enable extensile properties of active actin networks.Scientific reports · 2021Article
- Induced Arp2/3 Complex Depletion Increases FMNL2/3 Formin Expression and Filopodia Formation.Frontiers in cell and developmental biology · 2021Article
- Initiation and disassembly of filopodia tip complexes containing VASP and lamellipodin.Molecular biology of the cell · 2020Article
- Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion.eLife · 2020Article
- A direct role for SNX9 in the biogenesis of filopodia.The Journal of cell biology · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Filopodia are actin-dependent finger-like structures that protrude from the plasma membrane. Actin filament barbed-end-binding proteins localized to filopodial tips are key to filopodial assembly. Two classes of barbed-end-binding proteins are formins and Ena/VASP proteins, and both classes have been localized to filopodial tips in specific cellular contexts. Here, we examine the filopodial roles of the FMNL formins and Ena/VASP proteins in U2OS cells. FMNL3 suppression reduces filopodial assembly by 90%, and FMNL3 is enriched at >95% of filopodial tips. Suppression of VASP or Mena (also known as ENAH) reduces filopodial assembly by >75%. However, VASP and Mena do not display consistent filopodial tip localization, but are enriched in focal adhesions (FAs). Interestingly, >85% of FMNL3-containing filopodia are associated with FAs. Two situations increase Ena/VASP filopodial localization: (1) expression of myosin-X, and (2) actively spreading cells. In spreading cells, filopodia often mark sites of nascent adhesions. Interestingly, VASP suppression in spreading cells causes a significant increase in adhesion assembly at filopodial tips. This work demonstrates that, in U2OS cells, Ena/VASP proteins play roles in filopodia beyond those at filopodial tips.This article has an associated First Person interview with the first author of the paper.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.