ArticleMolecular and cellular biology1987
Three Drosophila beta-tubulin sequences: a developmentally regulated isoform (beta 3), the testis-specific isoform (beta 2), and an assembly-defective mutation of the testis-specific isoform (B2t8) reveal both an ancient divergence in metazoan isotypes and structural constraints for beta-tubulin function.
Article in Molecular and cellular biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 93 citations in OpenAlex.
- An Ixodes scapularis cell line with a predominantly neuron-like phenotype.Experimental & applied acarology · 2015Article
- Expression of ribosomal protein L22e family members in Drosophila melanogaster: rpL22-like is differentially expressed and alternatively spliced.Nucleic acids research · 2011Article
- Deficiency screen identifies a novel role for beta 2 tubulin in salivary gland and myoblast migration in the Drosophila embryo.Developmental dynamics : an official publication of the American Association of Anatomists · 2009Article
- Cooperativity between the beta-tubulin carboxy tail and the body of the molecule is required for microtubule function.Cell motility and the cytoskeleton · 2008Article
- Axoneme beta-tubulin sequence determines attachment of outer dynein arms.Current biology : CB · 2008Article
- Article
- Structurally similar Drosophila alpha-tubulins are functionally distinct in vivo.Molecular biology of the cell · 1997Article
- Article
- The beta-tubulin genes of Drosophila auraria are arranged in a cluster.Current genetics · 1994Article
- Expression of beta 1 tubulin (beta Tub56D) in Drosophila testis stem cells is regulated by a short upstream sequence while intron elements guide expression in somatic cells.Molecular & general genetics : MGG · 1993Article
- Either alpha-tubulin isogene product is sufficient for microtubule function during all stages of growth and differentiation in Aspergillus nidulans.Molecular and cellular biology · 1993Article
- Are tubulin isotypes functionally significant.Molecular biology of the cell · 1993Review
- The small genome of Arabidopsis contains at least nine expressed beta-tubulin genes.The Plant cell · 1992Article
- Microtubule behavior during guidance of pioneer neuron growth cones in situ.The Journal of cell biology · 1991Article
- A codon change in beta-tubulin which drastically affects microtubule structure in Drosophila melanogaster fails to produce a significant phenotype in Saccharomyces cerevisiae.Molecular and cellular biology · 1991Article
- The beta-tubulin gene family in Zea mays: two differentially expressed beta-tubulin genes.Plant molecular biology · 1990Article
- Two Drosophila beta tubulin isoforms are not functionally equivalent.The Journal of cell biology · 1990Article
- Molecular basis for determining the sensitivity of eucaryotes to the antimitotic drug rhizoxin.Molecular & general genetics : MGG · 1990Article
- Genetic and molecular analysis of a Caenorhabditis elegans beta-tubulin that conveys benzimidazole sensitivity.The Journal of cell biology · 1989Article
- The major component of the paraflagellar rod of Trypanosoma brucei is a helical protein that is encoded by two identical, tandemly linked genes.The Journal of cell biology · 1989Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
The genomic DNA sequence and deduced amino acid sequence are presented for three Drosophila melanogaster beta-tubulins: a developmentally regulated isoform beta 3-tubulin, the wild-type testis-specific isoform beta 2-tubulin, and an ethyl methanesulfonate-induced assembly-defective mutation of the testis isoform, B2t8. The testis-specific beta 2-tubulin is highly homologous to the major vertebrate beta-tubulins, but beta 3-tubulin is considerably diverged. Comparison of the amino acid sequences of the two Drosophila isoforms to those of other beta-tubulins indicates that these two proteins are representative of an ancient sequence divergence event which at least preceded the split between lines leading to vertebrates and invertebrates. The intron/exon structures of the genes for beta 2- and beta 3-tubulin are not the same. The structure of the gene for the variant beta 3-tubulin isoform, but not that of the testis-specific beta 2-tubulin gene, is similar to that of vertebrate beta-tubulins. The mutation B2t8 in the gene for the testis-specific beta 2-tubulin defines a single amino acid residue required for normal assembly function of beta-tubulin. The sequence of the B2t8 gene is identical to that of the wild-type gene except for a single nucleotide change resulting in the substitution of lysine for glutamic acid at residue 288. This position falls at the junction between two major structural domains of the beta-tubulin molecule. Although this hinge region is relatively variable in sequence among different beta-tubulins, the residue corresponding to glu 288 of Drosophila beta 2-tubulin is highly conserved as an acidic amino acid not only in all other beta-tubulins but in alpha-tubulins as well.
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