Evidence map›Paper›PMID 30373389›Full record

ArticleAnnals of clinical biochemistry2019

A rapid and sensitive fluorescence method for detecting urine formaldehyde in patients with Alzheimer's disease.

Li Ai, Jun Wang, Tingting Li, Chang Zhao, Yonghe Tang, Weishan Wang, Shengjie Zhao, Wenjing Jiang, Yalan Di, Xuechao Fei and 7 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of clinical biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 7 institutions in 1 country.

Li Ai1 Laboratory of Alzheimer's Optoelectric Therapy, Alzheimer's Disease Center, Beijing Institute of Brain Disorders, Center for Brain Disorders Research, Capital Medical University, Beijing, China.
Jun Wang2 Beijing No. 12 Laboratory of Brain and Cognitive Sciences, Beijing, China.
Tingting Li2 Beijing No. 12 Laboratory of Brain and Cognitive Sciences, Beijing, China.
Chang Zhao3 Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Shandong, China.
Yonghe Tang3 Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Shandong, China.
Weishan Wang4 Center for Cognitive Disorders, Beijing Geriatric Hospital, Beijing, China.
Shengjie Zhao5 Beijing Boai Hospital, China Rehabilitation Research Center, Beijing, China.
Wenjing Jiang1 Laboratory of Alzheimer's Optoelectric Therapy, Alzheimer's Disease Center, Beijing Institute of Brain Disorders, Center for Brain Disorders Research, Capital Medical University, Beijing, China.
Yalan Di1 Laboratory of Alzheimer's Optoelectric Therapy, Alzheimer's Disease Center, Beijing Institute of Brain Disorders, Center for Brain Disorders Research, Capital Medical University, Beijing, China.
Xuechao Fei1 Laboratory of Alzheimer's Optoelectric Therapy, Alzheimer's Disease Center, Beijing Institute of Brain Disorders, Center for Brain Disorders Research, Capital Medical University, Beijing, China.
Hongjun Luo6 Central Laboratory, Shantou University Medical College, Guangdong, China.
Hui Li6 Central Laboratory, Shantou University Medical College, Guangdong, China.
Wenhong Luo6 Central Laboratory, Shantou University Medical College, Guangdong, China.
Yan Yu5 Beijing Boai Hospital, China Rehabilitation Research Center, Beijing, China.
Weiying Lin3 Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Shandong, China.
Rongqiao He1 Laboratory of Alzheimer's Optoelectric Therapy, Alzheimer's Disease Center, Beijing Institute of Brain Disorders, Center for Brain Disorders Research, Capital Medical University, Beijing, China.
Zhiqian Tong1 Laboratory of Alzheimer's Optoelectric Therapy, Alzheimer's Disease Center, Beijing Institute of Brain Disorders, Center for Brain Disorders Research, Capital Medical University, Beijing, China.
Chinese Institute for Brain Research · CNUniversity of Jinan · CNBeijing Geriatric Hospital · CNCapital Medical University · CNChina Rehabilitation Research Center · CNShantou University · CNShantou University Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMorning urine formaldehyde concentrations could predict the severe degree of dementia in patients with post-stroke dementia and Alzheimer's disease. However, the routinely available technique of high-performance liquid chromatography (HPLC) for detecting urine formaldehyde requires expensive and sophisticated equipment.

methodsWe established a fluorescence spectrophotometric method by using a formaldehyde-specific fluorescent probe-NaFA (λex/em = 430/543 nm). As a standard reference method, the same batch of urine samples was analysed by HPLC with a fluorescence detector (λex/em = 346/422 nm). Then we compared the limits of detection and the limits of quantization detected by these two methods and addressed the relationship between urine formaldehyde and human cognitive ability. The Mini-Mental State Examination (MMSE), Clinical Dementia Rating and Activities of Daily Living scale were used to evaluate cognition function in 30 Alzheimer's disease patients and 52 healthy age-matched controls.

resultsLimits of detection and limits of quantization (1.27 and 2.48 μM) of the NaFA probe method were more accurate than Fluo-HPLC (1.52 and 2.91 μM). There was no difference in the detected formaldehyde values within day and day-to-day. Notably, only 3/82 urine formaldehyde concentrations detected by NaFA probe were below zero, while 12/82 of the values analysed by Fluo-HPLC were abnormal. More importantly, there were negatively correlated between urine formaldehyde concentrations detected by NaFA probe and MMSE scores, but positively correlated with Clinical Dementia Rating scores in Alzheimer's disease patients.

conclusionsThis detecting urine formaldehyde method by NaFA probe was more rapid, sensitive and accurate than Fluo-HPLC.

Indexed as

Limit of DetectionActivities of Daily LivingAgedAlzheimer DiseaseCase-Control StudiesCognitionFemaleFormaldehydeHumansMaleSpectrometry, FluorescenceTime FactorsUrinalysisFormaldehydeAlzheimer’s diseasedementiaFormaldehydehigh-performance liquid chromatographyNaFAspectrophotometry

Identifiers

PMID30373389
PMCPMC6415487
OpenAlexW2899324070

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.