Evidence map›Paper›PMID 30373006›Full record

ArticleFood chemistry2019

Peptidomic profiling of human milk with LC-MS/MS reveals pH-specific proteolysis of milk proteins.

Junai Gan, Randall C Robinson, Jiaqi Wang, Nithya Krishnakumar, Courtney J Manning, Yi Lor, Melissa Breck, Daniela Barile, J Bruce German

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Food chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
  2. Article
  3. UsingGut microbes reports · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. HBFP: a new repository for human body fluid proteome.Database : the journal of biological databases and curation · 2021
    Article
  10. Article
  11. Article
  12. Article
  13. Peptidomics and Capillary Electrophoresis.Advances in experimental medicine and biology · 2021
    Article
  14. Article
  15. Article
  16. Review
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Junai GanDepartment of Food Science and Technology, University of California Davis, USA. Electronic address: jagan@ucdavis.edu.
Randall C RobinsonDepartment of Food Science and Technology, University of California Davis, USA. Electronic address: rcrobinson@ucdavis.edu.
Jiaqi WangDepartment of Food Science and Technology, University of California Davis, USA. Electronic address: jqqwang@ucdavis.edu.
Nithya KrishnakumarDepartment of Food Science and Technology, University of California Davis, USA. Electronic address: nkumar@ucdavis.edu.
Courtney J ManningDepartment of Chemistry, University of California Davis, USA. Electronic address: cjmanning@ucdavis.edu.
Yi LorCollege of Biological Sciences, University of California Davis, USA. Electronic address: yijlor@ucdavis.edu.
Melissa BreckDepartment of Food Science and Technology, University of California Davis, USA; Foods for Health Institute, University of California Davis, USA. Electronic address: mabreck@ucdavis.edu.
Daniela BarileDepartment of Food Science and Technology, University of California Davis, USA; Foods for Health Institute, University of California Davis, USA. Electronic address: dbarile@ucdavis.edu.
J Bruce GermanDepartment of Food Science and Technology, University of California Davis, USA; Foods for Health Institute, University of California Davis, USA. Electronic address: jbgerman@ucdavis.edu.

Funding

Activation of probiotic bifidobacteria by milk glyansR01AT008759 · NCCIH · UNIVERSITY OF CALIFORNIA AT DAVIS · PI GERMAN, BRUCE, LEBRILLA, CARLITO B · 2014 to 2018
$4.2M
Mechanism of Activation of Probiotic Bifidobacteria by Prebiotic Milk GlycansR01AT007079 · NCCIH · UNIVERSITY OF CALIFORNIA AT DAVIS · PI GERMAN, BRUCE, LEBRILLA, CARLITO B · 2012 to 2016
$2.2M
NCCIH NIH HHS R01 AT007079NCCIH NIH HHS R01 AT008759
6 · The paper itself

Abstract

Human milk is a dynamic protein-protease system that delivers bioactive peptides to infants. The pH of milk changes from the mother's mammary gland to the infant's digestive tract. Although the release of human milk peptides has been studied during in vivo or in vitro digestion, these models did not explicitly vary nor observe the effect of pH. The objective of this research was to determine the effect of pH on the proteolysis of human milk. Using high-resolution accurate-mass Orbitrap mass spectrometry, profiles of endogenous human milk peptides before and after incubation at various pH levels have been mapped. Over 5000 peptides were identified. Comparative analyses classified 74 peptides that were consistently found independent of pH alterations, and 8 peptides that were released only at pH 4 or 5 (typical infant gastric pH). Results documented that the proteolysis of milk proteins, particularly β-casein, polymeric immunoglobulin receptor, and α-lactalbumin, is pH-dependent.

Indexed as

ProteolysisProteomicsAnimalsChromatography, LiquidFemaleHumansHydrogen-Ion ConcentrationMilk, HumanMilk ProteinsTandem Mass SpectrometryMilk ProteinsBioactive peptidesHuman milkMass spectrometryMilk peptidesPeptidomicsProtein digestionProteolysis

Identifiers

PMID30373006
PMCPMC6432908

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.