ArticlePloS one2018
Ascophyllum nodosum extract mitigates salinity stress in Arabidopsis thaliana by modulating the expression of miRNA involved in stress tolerance and nutrient acquisition.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- A Global Network Meta-Analysis of the Promotion of Crop Growth, Yield, and Quality by Bioeffectors.Frontiers in plant science · 2022Pooled it
- Emerging Trends in Nanotechnology and AI-Driven Valorization of Agro-Industrial Waste in Circular Bioeconomy for Production of Biostimulants.Foods (Basel, Switzerland) · 2026Review
- Ascophyllum nodosum based plant biostimulant shapes the bacterial community in the rhizosphere of corn.BMC plant biology · 2025Article
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- Genome-wide molecular evolution analysis of the GRF and GIF gene families in Plantae (Archaeplastida).BMC genomics · 2024Article
- Improvement of rooting and growth in kiwifruit (Heliyon · 2023Article
- A Plant Biostimulant fromPlants (Basel, Switzerland) · 2023Article
- Brown Seaweed Extract (BSE) Application Influences Auxin- and ABA-Related Gene Expression, Root Development, and Sugar Yield inPlants (Basel, Switzerland) · 2023Article
- Understanding the mode of action of AgroGainFrontiers in plant science · 2023Article
- A biostimulant prepared from red seaweedFrontiers in plant science · 2023Article
- Genome-wide analysis and identification of microRNAs inFrontiers in plant science · 2023Article
- Integrated analysis of long non-coding RNAs and mRNAs reveals the regulatory network of maize seedling root responding to salt stress.BMC genomics · 2022Article
- Strategies and prospects for biostimulants to alleviate abiotic stress in plants.Frontiers in plant science · 2022Review
- Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid inInternational journal of molecular sciences · 2021Article
- Seaweed-Based Compounds and Products for Sustainable Protection against Plant Pathogens.Marine drugs · 2021Review
- Article
- Application of Brown Algae (Sargassum angustifolium) Extract for Improvement of Drought Tolerance in Canola (Brassica napus L.).Iranian journal of biotechnology · 2021Article
- Transcriptomics of Biostimulation of Plants Under Abiotic Stress.Frontiers in genetics · 2021Review
- Reducing Nitrogen Input in Barley Crops While Maintaining Yields Using an Engineered Biostimulant Derived FromFrontiers in plant science · 2021Article
- Enhancing Sustainability by Improving Plant Salt Tolerance through Macro- and Micro-Algal Biostimulants.Biology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ascophyllum nodosum extract (ANE) contains bioactive compounds that improve the growth of Arabidopsis in experimentally-induced saline conditions; however, the molecular mechanisms through which ANE elicits tolerance to salinity remain largely unexplored. Micro RNAs (miRNAs) are key regulators of gene expression, playing crucial roles in plant growth, development, and stress tolerance. Next generation sequencing of miRNAs from leaves of control Arabidopsis and from plants subjected to three treatments (ANE, NaCl and ANE+NaCl) was used to identify ANE-responsive miRNA in the absence and presence of saline conditions. Differential gene expression analysis revealed that ANE had a strong effect on miRNAs expression in both conditions. In the presence of salinity, ANE tended to reduce the up-regulation or the down-regulation trend induced caused by NaCl in miRNAs such as ath-miR396a-5p, ath-miR399, ath-miR2111b and ath-miR827. To further uncover the effects of ANE, the expression of several target genes of a number of ANE-responsive miRNAs was analyzed by qPCR. NaCl, but not ANE, down-regulated miR396a-5p, which negatively regulated the expression of AtGRF7 leading to a higher expression of AtDREB2a and AtRD29 in the presence of ANE+NaCl, as compared to ANE alone. ANE+NaCl initially reduced and then enhanced the expression of ath-miR169g-5p, while the expression of the target genes AtNFYA1 and ATNFYA2, known to be involved in the salinity tolerance mechanism, was increased as compared to ANE or to NaCl treatments. ANE and ANE+NaCl modified the expression of ath-miR399, ath-miR827, ath-miR2111b, and their target genes AtUBC24, AtWAK2, AtSYG1 and At3g27150, suggesting a role of ANE in phosphate homeostasis. In vivo and in vitro experiments confirmed the improved growth of Arabidopsis in presence of ANE, in saline conditions and in phosphate-deprived medium, further substantiating the influence of ANE on a variety of essential physiological processes in Arabidopsis including salinity tolerance and phosphate uptake.
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