ArticleJournal of visualized experiments : JoVE2018
Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 69 citations in OpenAlex.
- Phosphoproteomic dysregulation promotes tumor proliferation in Cushing's disease.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Transcriptomic analysis of spinal V1 interneurons informs their multifunctional role in motor output.Nature communications · 2026Article
- Spinal signatures of entrenched and treated neuropathic pain in male mice.Nature communications · 2026Article
- Differential selectivity of microglia and astrocytes in HIV-1 gp120-induced synaptic pruning.Brain : a journal of neurology · 2026Article
- Article
- Identification of a reactive astrocyte subpopulation during HIV-associated pain pathogenesis in mouse models.Nature communications · 2025Article
- Variable Schwann cell merlin inactivation is targetable with TEAD1 inhibition in schwannomas.bioRxiv : the preprint server for biology · 2025Article
- Evolutionary expansion of the corticospinal system is linked to dexterity inbioRxiv : the preprint server for biology · 2025Article
- Co-Conservation of synaptic gene expression and circuitry in collicular neurons.Nature communications · 2025Article
- Innovations in spinal cord cell type heterogeneity across vertebrate evolution.bioRxiv : the preprint server for biology · 2025Article
- Fetal rat neural progenitor cell transplantation after spinal cord injury improves motor recovery following optogenetic stimulation.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Schizophrenia-associated 22q11.2 deletion elevates striatal acetylcholine and disrupts thalamostriatal projections to produce amotivation in mice.bioRxiv : the preprint server for biology · 2025Article
- An Open-Source Tool for Investigation of Differential RNA Expression Between Spinal Cord Cells of Male and Female Mice.Journal of neuroscience research · 2024Article
- The transcriptomic landscape of spinal V1 interneurons reveals a role for En1 in specific elements of motor output.bioRxiv : the preprint server for biology · 2024Article
- A conserved cell-type gradient across the human mediodorsal and paraventricular thalamus.bioRxiv : the preprint server for biology · 2024Article
- Synaptic plasticity in human thalamocortical assembloids.Cell reports · 2024Article
- Single-cell analysis of innate spinal cord regeneration identifies intersecting modes of neuronal repair.Nature communications · 2024Article
- Molecular Organization of Autonomic, Respiratory, and Spinally-Projecting Neurons in the Mouse Ventrolateral Medulla.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Heterogeneous fibroblasts contribute to fibrotic scar formation after spinal cord injury in mice and monkeys.Nature communications · 2024Article
- [Communications biology · 2024Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Probing an individual cell's gene expression enables the identification of cell type and cell state. Single-cell RNA sequencing has emerged as a powerful tool for studying transcriptional profiles of cells, particularly in heterogeneous tissues such as the central nervous system. However, dissociation methods required for single cell sequencing can lead to experimental changes in the gene expression and cell death. Furthermore, these methods are generally restricted to fresh tissue, thus limiting studies on archival and bio-bank material. Single nucleus RNA sequencing (snRNA-Seq) is an appealing alternative for transcriptional studies, given that it accurately identifies cell types, permits the study of tissue that is frozen or difficult to dissociate, and reduces dissociation-induced transcription. Here, we present a high-throughput protocol for rapid isolation of nuclei for downstream snRNA-Seq. This method enables isolation of nuclei from fresh or frozen spinal cord samples and can be combined with two massively parallel droplet encapsulation platforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.