ArticleSynthetic biology (Oxford, England)2018
Low-cost detection of norovirus using paper-based cell-free systems and synbody-based viral enrichment.
Article in Synthetic biology (Oxford, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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Who cites it
51 citing papers in PubMed, 134 citations in OpenAlex.
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- Automated and Programmable Cell-Free Systems for Scalable Synthetic Biology with a Focus on Biofoundry Integration.Journal of microbiology and biotechnology · 2025Review
- Gene circuit-based sensors.Fundamental research · 2025Review
- Detection of Murine Norovirus on Fresh Produce Through a CRISPR/Cas13a RNase-Based Capsid Integrity Assay.Food and environmental virology · 2025Article
- Detection of TuMV by a toehold switch sensor coupled with NASBA amplification in Pseudostellaria heterophylla.Plant methods · 2025Article
- Synthetic cell preservation strategies enable their storage and activation at the point of use.Chemical communications (Cambridge, England) · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Improved RNA toehold switch sensitivity using the NanoLuc complementation reporter.microPublication biology · 2025Article
- A Novel Approach Using LuxSit-i Enhanced Toehold Switches for the Rapid Detection ofBiosensors · 2024Article
- Regulatory Components for Bacterial Cell-Free Systems Engineering.ACS synthetic biology · 2024Review
- Article
- Detection of viral RNAs at ambient temperature via reporter proteins produced through the target-splinted ligation of DNA probes.Nature biomedical engineering · 2023Article
- Toehold switch plus signal amplification enables rapid detection.Biotechnology journal · 2023Article
- Plasmid Crosstalk in Cell-Free Expression Systems.ACS synthetic biology · 2023Article
- At-Home, Cell-Free Synthetic Biology Education Modules for Transcriptional Regulation and Environmental Water Quality Monitoring.ACS synthetic biology · 2023Article
- Streamlining the Detection of Human Thyroid Receptor Ligand Interactions with XL1-Blue Cell-Free Protein Synthesis and Beta-Galactosidase Fusion Protein Biosensors.Life (Basel, Switzerland) · 2023Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- Orthogonal Light-Activated DNA for Patterned Biocomputing within Synthetic Cells.Journal of the American Chemical Society · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Noroviruses are a primary cause of gastroenteritis and foodborne illness with cases that affect millions of people worldwide each year. Inexpensive tests for norovirus that do not require sophisticated laboratory equipment are important tools for ensuring that patients receive timely treatment and for containing outbreaks. Herein, we demonstrate a low-cost colorimetric assay that detects norovirus from clinical samples by combining paper-based cell-free transcription-translation systems, isothermal amplification and virus enrichment by synbodies. Using isothermal amplification and cell-free RNA sensing with toehold switches, we demonstrate that the assay enables detection of norovirus GII.4 Sydney from stool down to concentrations of 270 aM in reactions that can be directly read by eye. Furthermore, norovirus-binding synbodies and magnetic beads are used to concentrate the virus and provide a 1000-fold increase in assay sensitivity extending its detection limit to 270 zM. These results demonstrate the utility of paper-based cell-free diagnostic systems for identification of foodborne pathogens and provide a versatile diagnostic assay that can be applied to the concentration, amplification and detection of a broad range of infectious agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.