ArticleProtein science : a publication of the Protein Society2019
Spectral and structural analysis of a red fluorescent protein from Acropora digitifera.
Article in Protein science : a publication of the Protein Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Structural Analysis of the Large Stokes Shift Red Fluorescent Protein tKeima.Molecules (Basel, Switzerland) · 2024Article
- Applications of fluorescence spectroscopy in protein conformational changes and intermolecular contacts.BBA advances · 2023Article
- Metal-Induced Fluorescence Quenching of Photoconvertible Fluorescent Protein DendFP.Molecules (Basel, Switzerland) · 2022Article
- Spectroscopic Analysis of Fe Ion-Induced Fluorescence Quenching of the Green Fluorescent Protein ZsGreen.Journal of fluorescence · 2021Article
- Spectroscopic and Structural Analysis of CuBiosensors · 2020Article
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3 authors.
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Abstract
Fluorescent proteins (FPs) possess a wide variety of spectral properties that make them of widespread interest as optical markers. These proteins can be applied as pH indicators or metal biosensors. The discovery and characterization of new fluorescent proteins is expected to further extend their application. Here, we report the spectral and structural analysis of a red fluorescent protein from Acropora digitifera (designated AdRed). This protein shows a tetrameric state and is red emitting, with excitation and emission maxima at 567 and 612 nm, respectively. Its crystal structure shows the tetrameric interface stabilized by hydrogen bonding and salt bridges. The electron density map of the chromophore, consisting of Asp66-Tyr67-Gly68, shows the decarboxylated side chain of Asp66. Ser223, located near the chromophore, has the role of bridging His202 and Glu221, and is part of the hydrogen bond network. Mutated AdRed with Cys148Ser reveals a blue shift in fluorescence excitation and emission. Our results provide insights into understanding the molecular function of AdRed and other FPs.
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