Evidence map›Paper›PMID 30368725›Full record

ArticleClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico2019

Antigen-loaded dendritic cells triggers a specific cytotoxic T lymphocytes immune response against hepatocellular carcinoma: in vitro study.

N E El-Ashmawy, E A El-Zamarany, E G Khedr, H A El-Bahrawy, O A El-Feky

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Article in Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

N E El-AshmawyDepartment of Biochemistry, Faculty of Pharmacy, Tanta University, El-Bahr Street, Tanta, 31527, El-Gharbiya, Egypt.
E A El-ZamaranyDepartment of Clinical Pathology, Faculty of Medicine, Tanta University, Tanta, Egypt.
E G KhedrDepartment of Biochemistry, Faculty of Pharmacy, Tanta University, El-Bahr Street, Tanta, 31527, El-Gharbiya, Egypt.
H A El-BahrawyDepartment of Biochemistry, Faculty of Pharmacy, Tanta University, El-Bahr Street, Tanta, 31527, El-Gharbiya, Egypt.
O A El-FekyDepartment of Biochemistry, Faculty of Pharmacy, Tanta University, El-Bahr Street, Tanta, 31527, El-Gharbiya, Egypt. dr_ola23@yahoo.com.ORCID http://orcid.org/0000-0002-2662-4765
Tanta University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHepatocellular carcinoma (HCC) is one of the common malignancies, characterized by poor response to conventional therapeutic options. Immunotherapy with dendritic cells (DCs)-vaccines is one of the most successful strategies used for the treatment of HCC. However, the methods applied in the preparation of antigen-loaded DCs are important factors for optimization of DCs vaccines. PURPOSE: The present study was conducted to investigate the effect of HCC-whole tumor cell lysate prepared using rapid repetitive freeze-thaw cycles on the immunogenicity of DCs and evaluate the ability of whole tumor cell lysate-pulsed DCs vaccine to induce a specific cytotoxic T lymphocytes (CTLs) response against HepG2 cell line.

methodsImmature DCs generated from peripheral blood monocytes were randomized into two groups: control DCs and whole tumor cell lysate-pulsed DCs. Phenotypic analysis of the DCs' cell maturation marker CD83 and co-stimulatory molecule CD86 was performed. HCC-specific cytotoxic activity of CD8

resultsLoading of DCs with necrotic whole cell lysate resulted in non-significant changes in DCs' expression of CD83, but a significant increase in expression of CD86. In addition, CD8

conclusionOur findings indicated that pulsation of DCs with whole tumor cell lysate prepared by repetitive freeze-thaw cycles could efficiently enhance the ability of DCs to induce proliferation and clonal expansion of CD8

Indexed as

Antigens, NeoplasmCarcinoma, HepatocellularCD8-Positive T-LymphocytesCells, CulturedDendritic CellsHep G2 CellsHumansIn Vitro TechniquesLiver NeoplasmsMonocytesT-Lymphocytes, CytotoxicAntigens, NeoplasmCD8 cellCell immunotherapyDendritic cellsHepatocellular carcinoma

Identifiers

PMID30368725
OpenAlexW2898419933

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.