ArticlePflugers Archiv : European journal of physiology2019
Regulation of autophagic and mitophagic flux during chronic contractile activity-induced muscle adaptations.
Article in Pflugers Archiv : European journal of physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Exercise-induced lysosomal adaptations in skeletal muscle.Sports medicine and health science · 2026Review
- Padua Days on Muscle and Mobility Medicine, March 25-29, 2025, Hotel Petrarca, Euganean Thermae, Italy: Program and Abstracts.European journal of translational myology · 2025Article
- Mitophagy is required to protect against excessive skeletal muscle atrophy following hindlimb immobilization.Journal of biomedical science · 2025Article
- Exercise, cancer, and the cardiovascular system: clinical effects and mechanistic insights.Basic research in cardiology · 2025Review
- Relevance of Cardiovascular Exercise in Cancer and Cancer Therapy-Related Cardiac Dysfunction.Current heart failure reports · 2024Review
- The influence of age, sex, and exercise on autophagy, mitophagy, and lysosome biogenesis in skeletal muscle.Skeletal muscle · 2022Article
- p53 regulates skeletal muscle mitophagy and mitochondrial quality control following denervation-induced muscle disuse.The Journal of biological chemistry · 2022Article
- The role of mitophagy in the mechanism of genioglossal dysfunction caused by chronic intermittent hypoxia and the protective effect of adiponectin.Sleep & breathing = Schlaf & Atmung · 2021Article
- Mitophagy in Human Diseases.International journal of molecular sciences · 2021Review
- Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice.International journal of molecular sciences · 2021Article
- Moderate, but Not Excessive, Training Attenuates Autophagy Machinery in Metabolic Tissues.International journal of molecular sciences · 2020Article
- Molecular Basis for the Therapeutic Effects of Exercise on Mitochondrial Defects.Frontiers in physiology · 2020Review
- Exercise is mitochondrial medicine for muscle.Sports medicine and health science · 2019Review
- Review
- SR CaThe Journal of general physiology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy and mitophagy are important for training-inducible muscle adaptations, yet it remains unclear how these systems are regulated throughout the adaptation process. Here, we studied autophagic and mitophagic flux in the skeletal muscles of Sprague-Dawley rats (300-500 g) exposed to chronic contractile activity (CCA; 3 h/day, 9 V, 10 Hz continuous, 0.1 ms pulse duration) for 1, 2, 5, and 7 days (N = 6-8/group). In order to determine the flux rates, colchicine (COL; 0.4 mg/ml/kg) was injected 48 h before tissue collection, and we evaluated differences of autophagosomal protein abundances (LC3-II and p62) between colchicine- and saline-injected animals. We confirmed that CCA resulted in mitochondrial adaptations, including improved state 3 respiration as early as day 1 in permeabilized muscle fibers, as well significant increases in mitochondrial respiratory capacity and marker proteins in IMF mitochondria by day 7. Mitophagic and autophagic flux (LC3-II and p62) were significantly decreased in skeletal muscle following 7 days of CCA. Notably, the mitophagic system seemed to be downregulated prior (day 3-5) to changes in autophagic flux (day 7), suggesting enhanced sensitivity of mitophagy compared to autophagy with chronic muscle contraction. Although we detected no significant change in the nuclear translocation of TFEB, a regulator of lysosomal biogenesis, CCA increased total TFEB protein, as well as LAMP1, in skeletal muscle. Thus, chronic muscle activity reduces mitophagy in parallel with improved mitochondrial function, and this is supported by enhanced lysosomal degradation capacity.
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