Evidence map›Paper›PMID 30368557›Full record

ArticlePlanta2019

Characterization of genome-wide microRNAs and their roles in development and biotic stress in pear.

Qiulei Zhang, Yi Zhang, Shengnan Wang, Li Hao, Shengyuan Wang, Chaoran Xu, Feng Jiang, Tianzhong Li

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 20 citations in OpenAlex.

  1. miRNA-Mediated Regulation ofInternational journal of molecular sciences · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Qiulei ZhangLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
Yi ZhangLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
Shengnan WangLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
Li HaoLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
Shengyuan WangLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
Chaoran XuLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
Feng JiangLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China. jiangfeng@cau.edu.cn.
Tianzhong LiLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China. litianzhong1535@163.com.
China Agricultural University · CN

Funding

the Pear Modern Agroindustry Technology Research System CARS 28-08
6 · The paper itself

Abstract

MAIN

conclusionUsing a genome-wide analysis of miRNAs in 'Yali' pear (Pyrus bretschneideri) via the next-generation high-throughput sequencing of small RNAs with a bioinformatics analysis, we found that pbr-miR156, pbr-miR164, pbr-miR399, and pbr-miR482 and their target genes function in viral defense in 'Duli' and 'Hongbaoshi'. pbr-miR160, pbr-miR168, pbr-miR171, and pbr-miR319 and their targets function in auxin signaling pathways in 'Zhongai 4' and 'Zhongai 5'. Successful fruit production in pear (Pyrus spp.) depends on the use of optimal combinations of rootstocks and scions. Deciphering plant-pathogen defense mechanisms and hormone signaling pathways is an important step towards developing pear rootstocks and varieties with improved qualities. In the current study, we combined next-generation sequencing of small RNAs with a bioinformatics analysis to systematically identify and characterize 298 miRNAs in the pear scion cultivar 'Yali' (Pyrus bretschneideri). We also analyzed miRNAs in three rootstock varieties ('Duli', 'Zhongai 4', and 'Zhongai 5') and one scion cultivar ('Hongbaoshi'). We found that pbr-miR156, pbr-miR164, pbr-miR399, and pbr-miR482 are induced following infection with the pear virus Apple stem pitting virus (ASPV), and identified their target genes (pbRPS6, pbNAC, pbTLR, and pbRX-CC, respectively), which participate in viral defense pathways in 'Duli' and 'Hongbaoshi'. Furthermore, we identified pbr-miR160, pbr-miR168, pbr-miR171, and pbr-miR319, and found that the production of these miRNAs was suppressed under low levels of synthetic auxin. The targets of these miRNAs (pbARF, pbAEC, pbSCL, and pbTCP4) respond to auxin signaling pathways in 'Zhongai 4' and 'Zhongai 5'. Our results lay the foundation for breeding improved pear cultivars.

Indexed as

Cloning, MolecularFruitGenome-Wide Association StudyHigh-Throughput Nucleotide SequencingIndoleacetic AcidsMicroRNAsPlant Growth RegulatorsPyrusReal-Time Polymerase Chain ReactionRNA, PlantStress, PhysiologicalIndoleacetic AcidsMicroRNAsPlant Growth RegulatorsRNA, PlantBreedingDefenseDwarf rootstocksHormone signalingMicroRNAPyrusTarget genes

Identifiers

PMID30368557
OpenAlexW2898230231

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.