ArticleMethods in molecular biology (Clifton, N.J.)2019
Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis.
Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 citing papers in PubMed, 10 citations in OpenAlex.
- Muscle degeneration in aging Drosophila flies: the role of mechanical stress.Skeletal muscle · 2024Article
- The Role of Embryonic Chick Muscle Cell Culture in the Study of Skeletal Myogenesis.Frontiers in physiology · 2021Review
- An insight on Drosophila myogenesis and its assessment techniques.Molecular biology reports · 2020Review
- Regulation of fiber-specific actin expression by theDevelopment (Cambridge, England) · 2019Article
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6 authors at 2 institutions in 1 country.
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Abstract
Drosophila is a useful model organism for studying the molecular signatures that define specific muscle types during myogenesis. It possesses significant genetic conservation with humans for muscle disease causing genes and a lack of redundancy that simplifies functional analysis. Traditional molecular methods can be utilized to understand muscle developmental processes such as Western blots, in situ hybridizations, RT-PCR and RNAseq, to name a few. However, one challenge for these molecular methods is the ability to dissect different muscle types. In this protocol we describe some useful techniques for extracting muscles from the pupal and adult stages of development using flight and jump muscles as an example.
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