ArticleBotanical studies2018
Grain width 2 (GW2) and its interacting proteins regulate seed development in rice (Oryza sativa L.).
Article in Botanical studies, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 31 citations in OpenAlex.
- Mapping and validation of QTkw.cau-3DL, a major QTL controlling thousand-kernel weight in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Meta-QTL mapping for wheat thousand kernel weight.Frontiers in plant science · 2024Article
- Molecular Network for Regulation of Seed Size in Plants.International journal of molecular sciences · 2023Review
- QTL Analysis for Bread Wheat Seed Size, Shape and Color Characteristics Estimated by Digital Image Processing.Plants (Basel, Switzerland) · 2022Article
- A 24,482-bp deletion is associated with increased seed weight in Brassica napus L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Identification of novel QTLs for grain fertility and associated traits to decipher poor grain filling of basal spikelets in dense panicle rice.Scientific reports · 2021Article
- Genetic and Molecular Factors Determining Grain Weight in Rice.Frontiers in plant science · 2021Review
- RING-Type E3 Ubiqitin Ligase Barley Genes (Plants (Basel, Switzerland) · 2020Article
- SUPER STARCHY1/ONAC025 participates in rice grain filling.Plant direct · 2020Article
- Article
- A RING-Type E3 Ubiquitin Ligase,International journal of molecular sciences · 2020Article
- Silencing of an Ubiquitin Ligase Increases Grain Width and Weight inFrontiers in genetics · 2020Article
- Proteomic Data Integration Highlights Central Actors Involved in Einkorn (Frontiers in plant science · 2019Article
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Authors and funding
6 authors at 3 institutions in 1 country.
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Abstract
backgroundSeed size has been extensively studied in crop plants, as it determines crop yield. However, the mechanism of seed development remains elusive. In this study, we explored the mechanism of seed development in rice (Oryza sativa L.), and identified proteins affecting seed size.
resultsProteomic analysis showed that glyceraldehyde 3-phosphate dehydrogenase, chitinase 14 (CHT14), and phosphoglycerate kinase (PGK) accumulated to high levels in the seeds of the natural japonica rice mutant Oochikara, which carries a loss-of-function mutation in the grain width 2 (GW2) gene; GW2 encodes a RING-type E3 ubiquitin ligase. In vitro pull-down and ubiquitination assays showed that CHT14 and PGK directly interacted with GW2 but were not ubiquitinated by GW2. Immunoblot analysis revealed that protein disulfide isomerase-like 1-1 accumulated to high levels in young developing seeds of the gw2 mutant compared with the wild type. Histochemical β-glucuronidase staining showed strong expression of GW2 in leaf and root tissues but weak expression in leaf sheaths and internodes. In addition, transformation of the green fluorescent protein (GFP) gene under the control of the GW2 promoter in rice revealed GFP expression in the aleurone layer of seeds.
conclusionsCollectively, these results suggest that GW2 regulates seed size through direct interactions with proteins involved in carbohydrate metabolism by modulating their activity or stability and controlling disulfide bond formation in various proteins during seed development. Additionally, GW2 participates in vegetative as well as reproductive growth, and protects the seed from pathogen attack.
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