ArticleNature protocols2018
A practical guide to adaptive light-sheet microscopy.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Review
- A modular tissue-clearing framework integrated with light-field microscopy enables rapid volumetric phenotyping of cardiac tissue.Research square · 2026Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning.Intelligent computing (Washington, D.C.) · 2024Article
- Enhancing Light-Sheet Fluorescence Microscopy Illumination Beams through Deep Design Optimization.bioRxiv : the preprint server for biology · 2023Article
- Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy.The Journal of cell biology · 2023Review
- Deep learning-based adaptive optics for light sheet fluorescence microscopy.Biomedical optics express · 2023Article
- Maternal organophosphate flame retardant exposure alters the developing mesencephalic dopamine system in fetal rat.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- Practical considerations for quantitative light sheet fluorescence microscopy.Nature methods · 2022Review
- Active image optimization for lattice light sheet microscopy in thick samples.Biomedical optics express · 2022Article
- Challenges and advances in optical 3D mesoscale imaging.Journal of microscopy · 2022Article
- Quantitative analysis of illumination and detection corrections in adaptive light sheet fluorescence microscopy.Biomedical optics express · 2022Article
- Illumination angle correction during image acquisition in light-sheet fluorescence microscopy using deep learning.Biomedical optics express · 2022Article
- A Model of Discovery: The Role of Imaging Established and Emerging Non-mammalian Models in Neuroscience.Frontiers in molecular neuroscience · 2022Review
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- Deep learning-based autofocus method enhances image quality in light-sheet fluorescence microscopy.Biomedical optics express · 2021Article
- Towards a physical understanding of developmental patterning.Nature reviews. Genetics · 2021Review
- Single-objective high-resolution confocal light sheet fluorescence microscopy for standard biological sample geometries.Biomedical optics express · 2021Article
- Fluorescence based rapid optical volume screening system (OVSS) for interrogating multicellular organisms.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the implementation and use of an adaptive imaging framework for optimizing spatial resolution and signal strength in a light-sheet microscope. The framework, termed AutoPilot, comprises hardware and software modules for automatically measuring and compensating for mismatches between light-sheet and detection focal planes in living specimens. Our protocol enables researchers to introduce adaptive imaging capabilities in an existing light-sheet microscope or use our SiMView microscope blueprint to set up a new adaptive multiview light-sheet microscope. The protocol describes (i) the mechano-optical implementation of the adaptive imaging hardware, including technical drawings for all custom microscope components; (ii) the algorithms and software library for automated adaptive imaging, including the pseudocode and annotated source code for all software modules; and (iii) the execution of adaptive imaging experiments, as well as the configuration and practical use of the AutoPilot framework. Setup of the adaptive imaging hardware and software takes 1-2 weeks each. Previous experience with light-sheet microscopy and some familiarity with software engineering and building of optical instruments are recommended. Successful implementation of the protocol recovers near diffraction-limited performance in many parts of typical multicellular organisms studied with light-sheet microscopy, such as fruit fly and zebrafish embryos, for which resolution and signal strength are improved two- to fivefold.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.