ArticleNature communications2018
Enhanced mRNA FISH with compact quantum dots.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Dynamic Polymer Cross-linking Limits the Homogeneity of Compact Quantum Dots for Single-Particle Tracking.ACS applied materials & interfaces · 2025Article
- Intramolecular enhanced entropy-driven DNA-Au nanodevice for mRNA imaging in living cells.Mikrochimica acta · 2025Article
- Quantum Dot-Fab' Conjugates as Compact Immunolabels for Microtubule Imaging and Cell Classification.ACS nano · 2024Article
- Visualization of Individual RNA Molecules by Proximity Ligation-Based Chromogenic In Situ Hybridization Assay.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Digital and Absolute Assays for Low Abundance Molecular Biomarkers.Accounts of chemical research · 2023Article
- Review
- Structural Design of Multidentate Copolymers as Compact Quantum Dot Coatings for Live-Cell Single-Particle Imaging.Chemistry of materials : a publication of the American Chemical Society · 2022Article
- Antibody Self-Assembly Maximizes Cytoplasmic Immunostaining Accuracy of Compact Quantum Dots.Chemistry of materials : a publication of the American Chemical Society · 2021Article
- Engineering Brightness Matched Indium Phosphide Quantum Dots.Chemistry of materials : a publication of the American Chemical Society · 2021Article
- Ultra-bright Raman dots for multiplexed optical imaging.Nature communications · 2021Article
- Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Zwitterion and Oligo(ethylene glycol) Synergy Minimizes Nonspecific Binding of Compact Quantum Dots.ACS nano · 2020Article
- Short-Wave Infrared Quantum Dots with Compact Sizes as Molecular Probes for Fluorescence Microscopy.Journal of the American Chemical Society · 2020Article
- Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde.Mikrochimica acta · 2019Article
- Single molecule chromogenic in situ hybridization assay for RNA visualization in fixed cells and tissues.RNA (New York, N.Y.) · 2019Article
- Brief update on endocytosis of nanomedicines.Advanced drug delivery reviews · 2019Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Fluorescence in situ hybridization (FISH) is the primary technology used to image and count mRNA in single cells, but applications of the technique are limited by photophysical shortcomings of organic dyes. Inorganic quantum dots (QDs) can overcome these problems but years of development have not yielded viable QD-FISH probes. Here we report that macromolecular size thresholds limit mRNA labeling in cells, and that a new generation of compact QDs produces accurate mRNA counts. Compared with dyes, compact QD probes provide exceptional photostability and more robust transcript quantification due to enhanced brightness. New spectrally engineered QDs also allow quantification of multiple distinct mRNA transcripts at the single-molecule level in individual cells. We expect that QD-FISH will particularly benefit high-resolution gene expression studies in three dimensional biological specimens for which quantification and multiplexing are major challenges.
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Registered trials
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