ArticlePloS one2018
Involvement of VNUT-exocytosis in transient receptor potential vanilloid 4-dependent ATP release from gastrointestinal epithelium.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- The gut-brain axis and pain signalling mechanisms in the gastrointestinal tract.Nature reviews. Gastroenterology & hepatology · 2025Review
- Colonic TRPV4 overexpression is related to constipation severity.BMC gastroenterology · 2023Article
- SLC17A9 as a prognostic biomarker correlated with immune infiltrates in human non-small cell lung cancer.American journal of cancer research · 2023Article
- Potential Role of TRPV4 in Stretch-Induced Ghrelin Secretion and Obesity.International journal of endocrinology · 2022Article
- Quinacrine is not a vital fluorescent probe for vesicular ATP storage.Purinergic signalling · 2021Article
- Clodronate, an inhibitor of the vesicular nucleotide transporter, ameliorates steatohepatitis and acute liver injury.Scientific reports · 2021Article
- VNUT/SLC17A9, a vesicular nucleotide transporter, regulates osteoblast differentiation.FEBS open bio · 2020Article
- Polymodal Sensory Transduction in Mouse Corneal Epithelial Cells.Investigative ophthalmology & visual science · 2020Article
- Epithelial-Neuronal Communication in the Colon: Implications for Visceral Pain.Trends in neurosciences · 2020Review
- Review
- Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term.Heliyon · 2019Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adenosine triphosphate (ATP) modulates mechanosensitive vagal afferent nerves in the gastrointestinal tract. ATP is stored in secretory vesicles via the ATP transporter VNUT. Recently, the bisphosphate clodronate was reported to inhibit VNUT and was suggested to be a safe potent therapeutic option for chronic pain. Transient receptor potential vanilloid 4 (TRPV4) is activated by mechanical stimuli and some epoxyeicosatrienoic acids and becomes sensitized under inflammatory conditions. We have previously reported that TRPV4 and VNUT are expressed in mouse esophageal keratinocytes and that TRPV4 activation induces ATP release in gastric epithelial cells. Here we show the expression of TRPV4 and VNUT in normal human gastrointestinal cell derived cell lines (GES-1 and CCD 841) and in tissues from normal and VNUT-KO mice. TRPV4 agonists (GSK101 or 8,9-EET) induced an increase in cytosolic Ca2+ and/or current responses in mouse primary colonic epithelial cells and CCD 841 cells, but not in cells isolated from TRPV4-KO mice. TRPV4 agonists (GSK101 or 5.6-EET) also induced ATP release in GES-1 and CCD 841 cells, which could be blocked by the VNUT inhibitor, clodronate. Thus, VNUT inhibition with clodronate could represent a novel therapeutic option for visceral pain.
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