ArticleMolecular medicine reports2018
Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice.
Article in Molecular medicine reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Biologics for bone regeneration: advances in cell, protein, gene, and mRNA therapies.Bone research · 2026Review
- miR-144-3p targeting FLRT3 in osteogenic differentiation of mandibular bone marrow mesenchymal stem cells.Human genomics · 2025Article
- Extracellular vesicle-functionalized bioactive scaffolds for bone regeneration.Asian journal of pharmaceutical sciences · 2024Review
- Cell-based tissue engineered flexor tendon allograft: A canine in vivo study.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024Article
- Research progress in cell therapy for oral diseases: focus on cell sources and strategies to optimize cell function.Frontiers in bioengineering and biotechnology · 2024Review
- Review
- Exploring microRNAs in craniofacial regenerative medicine.Biochemical Society transactions · 2023Review
- Research Progress of Design Drugs and Composite Biomaterials in Bone Tissue Engineering.International journal of nanomedicine · 2023Review
- Clinical Perspectives of Non-Coding RNA in Oral Inflammatory Diseases and Neuropathic Pain: A Narrative Review.International journal of molecular sciences · 2022Review
- A Narrative Review of Cell-Based Approaches for Cranial Bone Regeneration.Pharmaceutics · 2022Review
- Research progress on the biological modifications of implant materials in 3D printed intervertebral fusion cages.Journal of materials science. Materials in medicine · 2021Review
- Roles of MicroRNAs in Osteogenesis or Adipogenesis Differentiation of Bone Marrow Stromal Progenitor Cells.International journal of molecular sciences · 2021Review
- Epigenetic reprogramming enhances the therapeutic efficacy of osteoblast-derived extracellular vesicles to promote human bone marrow stem cell osteogenic differentiation.Journal of extracellular vesicles · 2021Article
- Review
- An Overview of RNA-Based Scaffolds for Osteogenesis.Frontiers in molecular biosciences · 2021Review
- 3D Hybrid Nanofiber Aerogels Combining with Nanoparticles Made of a Biocleavable and Targeting Polycation and MiR-26a for Bone Repair.Advanced functional materials · 2020Article
- A Composite Tissue Engineered Bone Material Consisting of Bone Mesenchymal Stem Cells, Bone Morphogenetic Protein 9 (BMP9) Gene Lentiviral Vector, and P3HB4HB Thermogel (BMSCs-LV-BMP9-P3HB4HB) Repairs Calvarial Skull Defects in Rats by Expression of Osteogenic Factors.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- Exosomes derived from human CD34Stem cell research & therapy · 2019Article
- Roles for miRNAs in osteogenic differentiation of bone marrow mesenchymal stem cells.Stem cell research & therapy · 2019Review
- Adult Stem Cells for Bone Regeneration and Repair.Frontiers in cell and developmental biology · 2019Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Repair of bone defects presents a serious clinical challenge as it is difficult to restore bone function and regenerate bone loss. In the present study, the effects of lentivirus‑mediated transfection of bone marrow mesenchymal stem cells (BMSCs) with microRNA (miR)‑26a on bone regeneration were investigated in a mouse bone defect repair model. Marker of proliferation Ki67 (Ki67) staining was employed to detect the cell proliferation capacity and alkaline phosphatase (ALP) staining was used to investigate osteogenic differentiation. A mouse model of cranial bone defects was established. β‑tricalcium phosphate biomaterials co‑cultured with the transfected BMSCs were implanted into the defect areas of mouse models. Micro‑computed tomography, and hematoxylin and eosin and toluidine blue staining, were used to detect bone regeneration in the defect areas and the degradation of scaffolds. miR‑26a expression, and the mRNA and protein expression of osteogenesis‑associated cytokines, were detected using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Separated and cultured BMSCs highly expressed CD29 and CD105, but not CD34 and CD45, as determined by flow cytometry. miR‑26a expression and the positive cell rate of Ki67 and ALP staining in BMSCs transfected with pLVTHM‑miR‑26a were increased. The BMSC and negative control‑transfected BMSC groups exhibited increased bone regeneration in the defect areas, increased bone volume of newly formed bones, and elevated mRNA and protein expression of runt‑related transcription factor 2 (Runx2) and osteocalcin (OC), compared with the blank group. However, the miR‑26a‑transfected BMSC group exhibited further increases in bone regeneration and the volume of newly formed bones, and further elevations of the mRNA and protein expression levels of Runx2 and OC. The present findings demonstrated that lentivirus‑mediated modification of BMSCs enhanced bone regeneration during the repair of cranial bone defects in mice.
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