ArticleToxicological sciences : an official journal of the Society of Toxicology2019
Teratological and Behavioral Screening of the National Toxicology Program 91-Compound Library in Zebrafish (Danio rerio).
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 2 of them syntheses that pooled it.
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Who cites it
31 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- A Systematic Review to Compare Chemical Hazard Predictions of the Zebrafish Embryotoxicity Test With Mammalian Prenatal Developmental Toxicity.Toxicological sciences : an official journal of the Society of Toxicology · 2021Pooled it
- Toxicity testing of pesticides in zebrafish-a systematic review on chemicals and associated toxicological endpoints.Environmental science and pollution research international · 2020Pooled it
- Zebrafish behavioral assessment as a first-tier whole-organism NAM for developmental neurotoxicity: A multi-laboratory evaluation.Neurotoxicology · 2026Article
- Visual-acoustic thigmotaxis in zebrafish larvae: a high throughput NAM for neurotoxicity assessment.Frontiers in toxicology · 2026Article
- High-content toxicological profiling of 87 compounds using a 3D mouse mini-testis model: a New Approach Methodology (NAM) for prioritizing male reproductive toxicants.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Statistical analysis of multi-endpoint phenotypic screening increases sensitivity of planarian neurotoxicity testing.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
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- Utilizing Multiple Behavioral Endpoints to Identify Negative Control Chemicals in a Larval Zebrafish Behavior Assay.Toxics · 2025Article
- A data analysis approach for locomotor behavioral responses in developmentally exposed zebrafish.NAM journal · 2025Article
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- Influence of Methylene Blue or Dimethyl Sulfoxide on Larval Zebrafish Development and Behavior.Zebrafish · 2023Article
- Developmental Neurotoxicity Screen of Psychedelics and Other Drugs of Abuse in Larval Zebrafish (ACS chemical neuroscience · 2023Article
- An iron-chelating sulfonamide identified fromVirulence · 2022Article
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- Article
- Ryanodine receptor-active non-dioxin-like polychlorinated biphenyls cause neurobehavioral deficits in larval zebrafish.Frontiers in toxicology · 2022Article
- Zebrafish Embryos and Larvae as Alternative Animal Models for Toxicity Testing.International journal of molecular sciences · 2021Review
- Developmental phenotypic and transcriptomic effects of exposure to nanomolar levels of metformin in zebrafish.Environmental toxicology and pharmacology · 2021Article
- The efficacy of γ-aminobutyric acid type A receptor (GABAToxicology and applied pharmacology · 2021Article
- Zebrafish Models for the Safety and Therapeutic Testing of Nanoparticles with a Focus on Macrophages.Nanomaterials (Basel, Switzerland) · 2021Review
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Authors and funding
6 authors.
Funding
Abstract
To screen the tens of thousands of chemicals for which no toxicity data currently exists, it is necessary to move from in vivo rodent models to alternative models, such as zebrafish. Here, we used dechorionated Tropical 5D wild-type zebrafish embryos to screen a 91-compound library provided by the National Toxicology Program (NTP) for developmental toxicity. This library contained 86 unique chemicals that included negative controls, flame retardants, polycyclic aromatic hydrocarbons (PAHs), drugs, industrial chemicals, and pesticides. Fish were exposed to 5 concentrations of each chemical or an equal amount of vehicle (0.5% DMSO) in embryo medium from 6 h post-fertilization (hpf) to 5 days post-fertilization (dpf). Fish were examined daily for mortality and teratogenic effects and photomotor behavior was assessed at 4 and 5 dpf. Of the 5 negative control compounds in the library, none caused mortality/teratogenesis, but two altered behavior. Chemicals provided in duplicate produced similar outcomes. Overall, 13 compounds caused mortality/teratology but not behavioral abnormalities, 24 only affected behavior, and 18 altered both endpoints, with behavior affected at concentrations that did not cause mortality/teratology (55/86 hits). Of the compounds that affected behavior, 52% caused behavioral abnormalities at either 4 or 5 dpf. Compounds within the same functional group caused different behavioral abnormalities, while similar behavioral patterns were caused by compounds from different groups. Our data suggest that behavior is a sensitive endpoint for developmental toxicity screening that integrates multiple modes of toxic action and is influenced by the age of the larval fish at the time of testing.
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