Evidence map›Paper›PMID 30364590›Full record

ArticleMethodsX2018

Facial vein injection of human cells in severe combined immunodeficiency (SCID) neonatal mice.

Nimshitha Pavathuparambil Abdul Manaph, Mohammed Al-Hawwas, Liang Liu, Donghui Liu, John Hayball, Xin-Fu Zhou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MethodsX, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 88% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Recent Advances in In Vivo Somatic Cell Gene Modification in Newborn Pups.International journal of molecular sciences · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Nimshitha Pavathuparambil Abdul ManaphSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, Australia.
Mohammed Al-HawwasSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, Australia.
Liang LiuSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, Australia.
Donghui LiuSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, Australia.
John HayballSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, Australia.
Xin-Fu ZhouSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, Australia.
University of South Australia · AUChina Pharmaceutical University · CNRoyal Adelaide Hospital · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intravenous injection is a standard procedure for delivering human stem cells and therapeutic agents. Currently, genetically modified severe combined immunodeficiency (SCID) mice are used for engraftment studies using human cells. SCID neonates have better integration and survivability of human cells compared to adult SCID mice, as their immune system will not be developed in the first few days after birth. However, intravenous injections in neonates are difficult. This protocol describes a reliable and reproducible method for injecting cells into the facial vein of P3/P4 (3 or 4 days post-birth) SCID neonates to study their engraftment. The injection was safe and well tolerated by the pups. Post-injection analysis revealed the distribution of tagged cells in different organs. Results suggest that this new method can serve as a pre-analysis for transplantation studies using human stem cells before

Indexed as

DeliveryFacial veinFacial vein injection in P3/P4 pupsIntravenous injectionNeonatesStem cells

Identifiers

PMID30364590
PMCPMC6197144
OpenAlexW2895531838

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.