ArticleFrontiers in plant science2018
Respective Contributions of URT1 and HESO1 to the Uridylation of 5' Fragments Produced From RISC-Cleaved mRNAs.
Article in Frontiers in plant science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- RNA Tailing by Nucleotidyltransferases in Plants: Mechanisms, Functions, and Biological Significance.Plants (Basel, Switzerland) · 2026Review
- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- Unveiling the wheat NTP gene family: insights from genome-wide identification and TaNTP6 haplotype analysis.BMC plant biology · 2025Article
- The 3'-5' exoribonuclease ISG20L2 contributes to 3' terminus maturation of 18S and 28S ribosomal RNAs.Nucleic acids research · 2025Article
- Identification and Expression Analysis of the Nucleotidyl Transferase Protein (NTP) Family in Soybean (International journal of molecular sciences · 2024Article
- Functions and mechanisms of RNA tailing by nucleotidyl transferase proteins in plants.Frontiers in plant science · 2024Review
- An extensive survey of phytoviral RNA 3' uridylation identifies extreme variations and virus-specific patterns.Plant physiology · 2023Article
- Molecular mechanism underlying the di-uridylation activity of Arabidopsis TUTase URT1.Nucleic acids research · 2022Article
- Uridylation and the SKI complex orchestrate the Calvin cycle of photosynthesis through RNA surveillance ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- An insight into microRNA biogenesis and its regulatory role in plant secondary metabolism.Plant cell reports · 2022Review
- Catalytic activities, molecular connections, and biological functions of plant RNA exosome complexes.The Plant cell · 2022Review
- Mechanisms of MicroRNA Biogenesis and Stability Control in Plants.Frontiers in plant science · 2022Review
- Regulation of pri-MIRNA processing: mechanistic insights into the miRNA homeostasis in plant.Plant cell reports · 2021Review
- Regulation of RNA stability at the 3' end.Biological chemistry · 2021Review
- Prevalent cytidylation and uridylation of precursor miRNAs in Arabidopsis.Nature plants · 2019Article
- Article
- Plant microRNAs: Biogenesis, Homeostasis, and Degradation.Frontiers in plant science · 2019Review
- RNA uridylation and decay in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, post-transcriptional gene silencing (PTGS) represses gene expression by translation inhibition and cleavage of target mRNAs. The slicing activity is provided by argonaute 1 (AGO1), and the cleavage site is determined by sequence complementarity between the target mRNA and the microRNA (miRNA) or short interfering RNA (siRNA) loaded onto AGO1, to form the core of the RNA induced silencing complex (RISC). Following cleavage, the resulting 5' fragment is modified at its 3' end by the untemplated addition of uridines. Uridylation is proposed to facilitate RISC recycling and the degradation of the RISC 5'-cleavage fragment. Here, we detail a 3' RACE-seq method to analyze the 3' ends of 5' fragments produced from RISC-cleaved transcripts. The protocol is based on the ligation of a primer at the 3' end of RNA, followed by cDNA synthesis and the subsequent targeted amplification by PCR to generate amplicon libraries suitable for Illumina sequencing. A detailed data processing pipeline is provided to analyze nibbling and tailing at high resolution. Using this method, we compared the tailing and nibbling patterns of RISC-cleaved
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