ArticleInterventional medicine & applied science2018
Establishing methods for isolation of stem cells from human exfoliated deciduous from carious deciduous teeth.
Article in Interventional medicine & applied science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Bioenergetics of photobiomodulated osteoblast mitochondrial cells derived from human pulp stem cells: systematic review.Lasers in medical science · 2022Pooled it
- Nanoemulgel Development of Stem Cells from Human Exfoliated Deciduous Teeth-Derived Conditioned Medium as a Novel Nanocarrier Growth Factors.European journal of dentistry · 2026Article
- Cytotoxicity, DNA damage, and migration of SHEDs exposed to bioactive pulp capping materials: an in vitro study.BMC oral health · 2026Article
- Conditioned-medium of stem cells from human exfoliated deciduous teeth prevent apoptosis of neural progenitors.The Saudi dental journal · 2022Article
- Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration.International journal of molecular sciences · 2021Review
- Characteristics, Classification, and Application of Stem Cells Derived from Human Teeth.Stem cells international · 2021Review
- Pulp stem cells derived from human permanent and deciduous teeth: Biological characteristics and therapeutic applications.Stem cells translational medicine · 2020Review
- Response of stem cells from human exfoliated deciduous teeth (SHED) to three bioinductive materials - An in vitro experimental study.The Saudi dental journal · 2020Article
- The Circular RNA circRNA124534 Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Modulation of the miR-496/β-Catenin Pathway.Frontiers in cell and developmental biology · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimThis study aims to establish the isolation method of stem cells from pulp tissue of carious deciduous teeth.
methodsThe teeth were soaked in 1% povidone-iodine solution for about 1 min followed by washing in PBS with 1% antibiotic-antimycotic thrice. Dental pulp tissue was removed by extirpation, and then cultivated in the culture medium. Characterization of mesenchymal stem cell (MSC) was carried out using human MSC analysis kit with positive markers CD90, CD73, and CD105, but negative for expressions of CD45, CD34, CD11b, CD19, and HLA-DR. Differentiation capacity of stem cells from human exfoliated deciduous (SHED) was determined by staining with Alizarin S, Alcian Blue, and Oil Red O.
resultsThere is no contamination after 3 days of culture. SHED derived from dental pulp were expressions of 99.2% of positive marker and 0.3% of the negative marker. At passage 5, SHED was differentiated into osteocyte, chondrocyte, and adipocyte types of cells in the induction medium.
conclusionSHED derived from carious deciduous teeth can be used as a source of stem cell for regenerative medicine.
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