Evidence map›Paper›PMID 30362053›Full record

ArticleJournal of assisted reproduction and genetics2019

Altered three-dimensional organization of sperm genome in DPY19L2-deficient globozoospermic patients.

Fatma Abdelhedi, Céline Chalas, Jean-Maurice Petit, Nouha Abid, Elyes Mokadem, Syrine Hizem, Hassen Kamoun, Leila Keskes, Jean-Michel Dupont

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Fatma AbdelhediGénomique, Epigénétique et Physiopathologie de la Reproduction, U1016 INSERM-UMR 8104 CNRS, Institut Cochin, Université Paris Descartes, Paris, France. abdelhedi_f@yahoo.fr.ORCID http://orcid.org/0000-0001-5088-8384
Céline ChalasLaboratoire d'Histologie Embryologie - Biologie de la Reproduction, Hôpital Cochin, APHP, 75014, Paris, France.
Jean-Maurice PetitSorbonne Paris Cité, Université Paris Descartes, Paris, France.
Nouha AbidCentre d'Aide Médicale à la Procréation, Polyclinique Ibn Nafiss, Sfax, Tunisie.
Elyes MokademCentre d'Aide Médicale à la Procréation, Polyclinique El Alia, Sfax, Tunisie.
Syrine HizemLaboratoire de Cytogénétique, Hôpital Cochin, APHP, 75014, Paris, France.
Hassen KamounLaboratoire de Génétique Moléculaire Humaine, Faculté de Médecine de Sfax, Sfax, Tunisie.
Leila KeskesLaboratoire de Génétique Moléculaire Humaine, Faculté de Médecine de Sfax, Sfax, Tunisie.
Jean-Michel DupontGénomique, Epigénétique et Physiopathologie de la Reproduction, U1016 INSERM-UMR 8104 CNRS, Institut Cochin, Université Paris Descartes, Paris, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo explore the three-dimensional (3D) organization of sperm genome in DPY19L2-deficient globozoospermic patients speculating a link between DPY19L2 and genome organization of sperm nucleus.

methodsThis is a study of chromatin organization in DPY19L2-deficient globozoospermic patients and healthy donors using three-dimensional fluorescence in situ hybridization (3D-FISH) combined with confocal laser scanning microscopy followed by 3D image analysis. The 3D structures of sperm nuclei, chromocenter, telomeric regions and chromosome territories (CTs), were reconstructed using IMARIS software, and the relative radial position for each individual signal was calculated. Statistical analysis used a non-parametric Mann-Whitney test was appropriate with significance at p < 0.05.

resultsDPY19L2-deficient globozoospermic patients display impaired sperm chromocenter organization resulting in an increased number of chromocenters (5.4 vs 3.5; p < 0.0001). Moreover, radial positions of telomeres are modified with a more central position in globozoospermic nuclei. 3D-FISH analysis of five chromosome territories (CTs) (X, Y, 7, 17, 18) showed that DPY19L2-deficient globozoospermic sperm nuclei display altered spatial organization of CT X, CT 7 and CT 18.

conclusionsOur findings strengthen the hypothesis that DPY19L2 might be considered as a LINC-like protein having a crucial role in the organization of nuclear chromatin in sperm nucleus through its interaction with nuclear lamina. Our results might also explain defective embryonic development after intracytoplasmic sperm injection (ICSI) performed with DPY19L2-deficient globozoospermic sperm.

Indexed as

Genome, HumanAdultCase-Control StudiesChromosomes, HumanHumansIn Situ Hybridization, FluorescenceMaleMembrane ProteinsSpermatozoaTeratozoospermiaDPY19L2 protein, humanMembrane ProteinsChromatin organizationDPY19L2 geneGlobozoospermiaNuclear laminaThree-dimensional fluorescence in situ hybridization (3D-FISH)

Identifiers

PMID30362053
PMCPMC6338597

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.