Evidence map›Paper›PMID 30361813›Full record

ArticleChinese journal of integrative medicine2019

Paeoniflorin Inhibits Migration- and Invasion-Promoting Capacities of Gastric Cancer Associated Fibroblasts.

Zhen-Fei Wang, Da-Guang Ma, Ling Wang, Li Feng, Jian-Wei Fu, Ying Li, Dan-Ting Wang, Yong-Feng Jia

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In one paragraph

Article in Chinese journal of integrative medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.8field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 27 citations in OpenAlex.

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  5. The Role and Mechanism ofMolecules (Basel, Switzerland) · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Zhen-Fei WangThe Laboratory for Tumor Molecular Diagnosis, Affiliated People's Hospital of Inner Mongolia Medical University, Hohhot, 010020, China.
Da-Guang MaThe Laboratory for Tumor Molecular Diagnosis, Affiliated People's Hospital of Inner Mongolia Medical University, Hohhot, 010020, China.
Ling WangDepartment of Gastroenterology, Affiliated People's Hospital of Inner Mongolia Medical University, Hohhot, 010020, China.
Li FengDepartment of Gastroenterology, Affiliated People's Hospital of Inner Mongolia Medical University, Hohhot, 010020, China.
Jian-Wei FuThe Central Laboratory of People' Hospital of Wuhai City, Inner Mongolia Autonomous Region, Wuhai, 016000, China.
Ying LiDepartment of Gastroenterology, Affiliated People's Hospital of Inner Mongolia Medical University, Hohhot, 010020, China.
Dan-Ting WangDepartment of Gastroenterology, Affiliated People's Hospital of Inner Mongolia Medical University, Hohhot, 010020, China.
Yong-Feng JiaThe Laboratory for Tumor Molecular Diagnosis, Affiliated People's Hospital of Inner Mongolia Medical University, Hohhot, 010020, China. wzfsub12@126.com.
Inner Mongolia Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the inhibitory effects of paeoniflorin on migration- and invasion-promoting capacities of gastric cancer associated fibroblasts (GCAFs) and to explore the molecular mechanism underlying the effects.

methodsPaired gastric normal fifbroblast (GNF) and GCAF cultures were established from resected tissues. GCAFs were treated with control medium, or 2.5, 5 or 10 μg/mL paeoniflorin. Conditioned media were prepared from GNFs, GCAFs, control-treated GCAFs and paeoniflorin-treated GCAFs, and used to culture AGS human gastric cancer cells. The migration and invasion capacities of AGS cells were determined with wound healing test and transwell invasion assay, respectively. The interleukin 6 (IL-6) mRNA and microRNA-149 expression in GCAFs were detected by reverse transcription-quantitative polymerase chain reaction. The IL-6 protein expression and secretion by GCAFs were measured with Western blot and enzyme-linked immunosorbent assay analysis, respectively. The protein levels of phosphorylated signal transducer and activator of transcription 3 (STAT3), matrix metalloproteinase (MMP) and MMP9 in AGS cells were examined by Western blot.

resultsGCAFs displayed enhanced capacities to induce AGS cell migration and invasion as compared with GNFs. Paeoniflorin treatment significantly inhibited the migration- and invasion-promoting capacities of GCAFs (P<0.05). GCAFs produced and secreted more IL-6 into the conditioned medium than GNFs, leading to over-activation of STAT3-MMP signaling in AGS cells. Paeoniflorin suppressed IL-6 production and secretion by up-regulating microRNA149 expression in GCAFs, and subsequently prevented GCAFs from activating IL-6-STAT3-MMP signaling of AGS cells.

conclusionsPaeoniflorin inhibits the migration- and invasion-promoting capacities of GCAFs by targeting microRNA-149 and IL-6. Paeoniflorin is potentially a novel therapeutic agent against cancer microenvironment.

Indexed as

AdenocarcinomaAdultCancer-Associated FibroblastsCell MovementCells, CulturedCulture Media, ConditionedDown-RegulationFibroblastsGene Expression Regulation, NeoplasticGlucosidesGlycosidesHumansInterleukin-6MaleMargins of ExcisionMicroRNAsCulture Media, ConditionedGlucosidesGlycosidesIL6 protein, humanInterleukin-6MicroRNAsMIRN149 microRNA, humanMonoterpenespeoniflorinChinese medicinegastric cancer associated fibroblastsinvasionmigrationpaeoniflorin

Identifiers

PMID30361813
OpenAlexW2898135147

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.