Evidence map›Paper›PMID 30360761›Full record

ArticleGenome biology2018

Alternative polyadenylation factors link cell cycle to migration.

Mithun Mitra, Elizabeth L Johnson, Vinay S Swamy, Lois E Nersesian, David C Corney, David G Robinson, Daniel G Taylor, Aaron M Ambrus, David Jelinek, Wei Wang and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genome biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 47 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Mithun MitraDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
Elizabeth L JohnsonDepartment of Molecular Biology, Princeton University, Princeton, NJ, USA.
Vinay S SwamyDepartment of Biochemistry, University of California, Los Angeles, Los Angeles, CA, USA.
Lois E NersesianDepartment of Chemical Engineering, University of California, Los Angeles, Los Angeles, CA, USA.
David C CorneyDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
David G RobinsonLewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA.
Daniel G TaylorDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
Aaron M AmbrusDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
David JelinekDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
Wei WangLewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA.
Sandra L BatistaDepartment of Computer Science, University of Southern California, Los Angeles, CA, USA.
Hilary A CollerDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA. hcoller@ucla.edu.ORCID 0000-0003-0992-6494
University of California, Los Angeles · USPrinceton University · USUniversity of Southern California · US

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
Spatial Patterning During DevelopmentP50GM071508 · NIGMS · PRINCETON UNIVERSITY · PI POWELL, SUSAN M · 2004 to 2013
$30.6M
TUMOR CELL SURFACES AND CHROMOSOMEST32CA009056 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Gay M Crooks, David A. Nathanson · 1985 to 2026
$10.5M
Metabolic Control of Hair Follicle Stem Cell Homeostasis and TumorigenesisR01AR070245 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHRISTOFK, HEATHER, LOWRY, WILLIAM E · 2018 to 2022
$2.1M
The Role of Stromal Autophagy in Cutaneous MelanomaR01CA221296 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI COLLER, HILARY A · 2018 to 2022
$1.8M
The Role of MicroRNAs in Cellular QuiescenceR01GM081686 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI COLLER, HILARY A · 2008 to 2012
$1.4M
Dermatology Scientist Training ProgramT32AR071307 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MODLIN, ROBERT L · 2017 to 2021
$1.1M
NCATS NIH HHS UL1 TR000124NCI NIH HHS 1 R01 CA221296-01A1NCI NIH HHS P30 CA016042NCI NIH HHS R01 CA221296NCI NIH HHS T32 CA009056NIAMS NIH HHS AR070245NIAMS NIH HHS R01 AR070245NIAMS NIH HHS T32 AR071307NIAMS NIH HHS T32AR071307NIGMS NIH HHS GM081686NIGMS NIH HHS GM0866465NIGMS NIH HHS P50 GM071508NIGMS NIH HHS R01 GM081686NIH HHS UL1TR000124
6 · The paper itself

Abstract

backgroundIn response to a wound, fibroblasts are activated to migrate toward the wound, to proliferate and to contribute to the wound healing process. We hypothesize that changes in pre-mRNA processing occurring as fibroblasts enter the proliferative cell cycle are also important for promoting their migration.

resultsRNA sequencing of fibroblasts induced into quiescence by contact inhibition reveals downregulation of genes involved in mRNA processing, including splicing and cleavage and polyadenylation factors. These genes also show differential exon use, especially increased intron retention in quiescent fibroblasts compared to proliferating fibroblasts. Mapping the 3' ends of transcripts reveals that longer transcripts from distal polyadenylation sites are more prevalent in quiescent fibroblasts and are associated with increased expression and transcript stabilization based on genome-wide transcript decay analysis. Analysis of dermal excisional wounds in mice reveals that proliferating cells adjacent to wounds express higher levels of cleavage and polyadenylation factors than quiescent fibroblasts in unwounded skin. Quiescent fibroblasts contain reduced levels of the cleavage and polyadenylation factor CstF-64. CstF-64 knockdown recapitulates changes in isoform selection and gene expression associated with quiescence, and results in slower migration.

conclusionsOur findings support cleavage and polyadenylation factors as a link between cellular proliferation state and migration.

Indexed as

Cell CycleCell MovementPolyadenylationCells, CulturedFibroblastsHumansmRNA Cleavage and Polyadenylation FactorsPoly ARNA SplicingSkinmRNA Cleavage and Polyadenylation FactorsPoly AMigrationmRNA processingProliferationQuiescenceWound healing

Identifiers

PMID30360761
PMCPMC6203201
OpenAlexW2898173109

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.