Evidence map›Paper›PMID 30360508›Full record

ArticleToxins2018

Detection of Shiga Toxin 2 Produced by

Cheryl M Armstrong, Leah E Ruth, Joseph A Capobianco, Terence P Strobaugh, Fernando M Rubio, Andrew G Gehring

Abstract read
In one paragraph

Article in Toxins, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Genome Sequence Analysis and Characterization of Shiga Toxin 2 Production byFrontiers in cellular and infection microbiology · 2022
    Article
  12. Article
  13. Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Cheryl M ArmstrongMolecular Characterization of Foodborne Pathogens Research Unit, United States Department of Agriculture, Eastern Regional Research Center, Wyndmoor, PA 19038, USA. Cheryl.Armstrong@ars.usda.gov.ORCID 0000-0002-8553-9325
Leah E RuthAbraxis, Inc., Warminster, PA 18974, USA. lruth@abraxiskits.com.
Joseph A CapobiancoMolecular Characterization of Foodborne Pathogens Research Unit, United States Department of Agriculture, Eastern Regional Research Center, Wyndmoor, PA 19038, USA. Joseph.Capobianco@ars.usda.gov.ORCID 0000-0003-3651-4284
Terence P StrobaughMolecular Characterization of Foodborne Pathogens Research Unit, United States Department of Agriculture, Eastern Regional Research Center, Wyndmoor, PA 19038, USA. Terence.Strobaugh@ars.usda.gov.
Fernando M RubioAbraxis, Inc., Warminster, PA 18974, USA. frubio@abraxiskits.com.
Andrew G GehringMolecular Characterization of Foodborne Pathogens Research Unit, United States Department of Agriculture, Eastern Regional Research Center, Wyndmoor, PA 19038, USA. Andrew.Gehring@ars.usda.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA) is comprised of a bead-based immunoassay that is used for small molecule detection. In this study, a novel AlphaLISA was developed and optimized for the detection of Shiga-toxin 2 (Stx2). Efficacy and sensitivity trials showed the AlphaLISA could detect ≥0.5 ng/mL of purified Stx2, which was comparable to the industry-standard enzyme-linked immunosorbent assay (ELISA) tests for Stx2 detection. In addition, evaluation of Shiga toxin-producing

Indexed as

LactucaAntibodiesFood ContaminationImmunoassayLimit of DetectionPlant LeavesRed MeatShiga-Toxigenic Escherichia coliShiga Toxin 2AntibodiesShiga Toxin 2amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA)detectionE. colienzyme-linked immunosorbent assay (ELISA)Shiga toxinSTECStx2

Identifiers

PMID30360508
PMCPMC6265734

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.