ArticlePloS one2018
Rapid detection and differentiation of Salmonella species, Salmonella Typhimurium and Salmonella Enteritidis by multiplex quantitative PCR.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Analytical Validation and Preliminary Diagnostic Performance Evaluation of GenoPATHX™ Multiplex qPCR for Quantitative Detection of KeyPathogens (Basel, Switzerland) · 2026Article
- Exploring synergistic effect of bacteriophages with probiotics against multidrug resistantJournal of animal science and technology · 2026Article
- Conformation-sensitive nanobody immunoassay enables differentiation of live and heat-inactivated Salmonella Typhimurium for food safety monitoring.Food chemistry · 2026Article
- Application of CRISPR/Cas13a system on the rapid detection of Salmonella spp.PLoS neglected tropical diseases · 2026Article
- Child exposure to animal feces and zoonotic pathogens in northwest Ecuador: A mixed-methods study.PLoS neglected tropical diseases · 2026Article
- Combination of bacteriophage-probiotics alleviates intestinal barrier dysfunction by regulating gut microbiome in a chick model of multidrug-resistant Salmonella infection.Journal of animal science and biotechnology · 2026Article
- Rapid and low-cost detection of six major pediatric enteric pathogens using a closed-tube PCR-invasive reaction assay.Frontiers in cellular and infection microbiology · 2026Article
- High-throughput multiplex qPCR for distinguishing Salmonella serovars Heidelberg, Enteritidis, Typhimurium, Gallinarum, and Pullorum.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- A Novel Loop-Mediated Isothermal Amplification (LAMP) Assay for Detecting Salmonella Ser. Typhimurium in Egg Products.Journal of AOAC International · 2025Article
- CRISPR-Responsive Reprogrammable Label-Free Fluorescent Nanoclusters for ML-Assisted Pathogenic Genome Detection on Solid Substrates.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Accumulation of microbial hazards and assessment of food hygiene associated with broiler chicken processing at open air food markets in Maputo, Mozambique.International journal of food microbiology · 2025Article
- Prevalence of Shiga toxin-producing Escherichia coli, Salmonella, and Campylobacter species among diarrheal patients from three major hospitals in Ethiopia.PLOS global public health · 2025Article
- Toward same-day detection ofFrontiers in microbiology · 2025Article
- Multiplex PCR-based genotyping of Salmonella Enteritidis and Salmonella Typhimurium from food sources and assessment of their antimicrobial resistance profiles in Egypt.Molecular biology reports · 2024Article
- Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics.Future microbiology · 2024Review
- Article
- Review of Detection Limits for Various Techniques for Bacterial Detection in Food Samples.Nanomaterials (Basel, Switzerland) · 2024Review
- Establishment of a Simple, Sensitive, and SpecificFoods (Basel, Switzerland) · 2024Article
- Antibiotic Resistance Profile ofMicroorganisms · 2024Article
- Development of rapid nucleic acid testing techniques for common respiratory infectious diseases in the Chinese population.Frontiers in chemistry · 2024Article
Corrections and comments
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Authors and funding
7 authors.
Funding
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Abstract
A multiplex quantitative PCR (qPCR) was developed and evaluated for the simultaneous detection of Salmonella spp., S. enterica serovar Typhimurium and S. enterica serovar Enteritidis in various (food) matrices. Early and fast detection of these pathogens facilitates effective intervention and prevents further distribution of contaminated food products on the market. Three primer and probe sets were designed to target the invA gene, the STM4200 gene, and the SEN1392 gene to detect and differentiate Salmonella spp., S. Typhimurium, and S. Enteritidis, respectively. The multiplex qPCR targeting these three genes was optimized for efficiency and linearity. By testing 225 Salmonella isolates and 34 non-Salmonella isolates from various sources the inclusivity and exclusivity were determined. The inclusivity of the multiplex qPCR was 100% for all Salmonella isolates, including 72 S. Typhimurium isolates, and 53 S. Enteritidis isolates. The exclusivity for Salmonella spp., S. Typhimurium, and S. Enteritidis was 100%, 94.6%, and 100%, respectively. No positive results were reported for non-Salmonella isolates. The limit of detection (LOD) for the qPCR was determined for the matrices poultry, minced meat, egg, herbs/spices, powdered milk, fish, animal feed, boot-socks with chicken feces and chicken down. LOD values for qPCR and the conventional culture methods were similar, except for the matrix boot-socks and down, for which the LOD for the conventional culture methods performed better than the qPCR method. In conclusion, the multiplex qPCR assay developed allows for rapid screening of Salmonella spp., S. Typhimurium, and S. Enteritidis in various (food) matrices.
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